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  5. IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression

IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression
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Designed for high-level expression of recombinant proteins in Yeast by introducing the gene of interest under the control of the Cu++-inducible CUP1 promoter.

220.00 - 266.00

Specificatie

Indeling Liquid
Voor gebruik met (apparatuur) StarGate™ System, Yeast Host Cells
Inducerend middel Copper sulfate
Markergen leu2-d and URA3
pH 8
Meer specificaties weergeven

Producten 21
Produktcode Merk Vector Prijs Hoeveelheid en beschikbaarheid  
Produktcode Merk Vector Prijs Hoeveelheid en beschikbaarheid  
15613808
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IBA Lifesciences
5-4600-001 		pYSG-IBA wt1 N/A N/A N/A
15623808
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IBA Lifesciences
5-4603-001 		pYSG-IBA 3 N/A N/A N/A
15633808
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IBA Lifesciences
5-4605-001 		pYSG-IBA 5 N/A N/A N/A
15643808
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IBA Lifesciences
5-4623-001 		pYSG-IBA 23 N/A N/A N/A
15653808
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IBA Lifesciences
5-4625-001 		pYSG-IBA 25 N/A N/A N/A
15663808
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IBA Lifesciences
5-4633-001 		pYSG-IBA 33 N/A N/A N/A
15673808
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IBA Lifesciences
5-4643-001 		pYSG-IBA 43 N/A N/A N/A
15683808
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IBA Lifesciences
5-4645-001 		pYSG-IBA 45 N/A N/A N/A
15693808
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IBA Lifesciences
5-4662-001 		pYSG-IBA 62 N/A N/A N/A
15603818
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IBA Lifesciences
5-4663-001 		pYSG-IBA 63 N/A N/A N/A
15613818
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IBA Lifesciences
5-4664-001 		pYSG-IBA 64 N/A N/A N/A
15623818
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IBA Lifesciences
5-4665-001 		pYSG-IBA 65 N/A N/A N/A
15633818
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IBA Lifesciences
5-4703-001 		pYSG-IBA 103 N/A N/A N/A
15643818
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IBA Lifesciences
5-4705-001 		pYSG-IBA 105 N/A N/A N/A
15653818
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IBA Lifesciences
5-4723-001 		pYSG-IBA 123 N/A N/A N/A
15663818
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IBA Lifesciences
5-4743-001 		pYSG-IBA 143 N/A N/A N/A
15673818
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IBA Lifesciences
5-4745-001 		pYSG-IBA 145 N/A N/A N/A
15683818
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IBA Lifesciences
5-4762-001 		pYSG-IBA 162 N/A N/A N/A
15693818
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IBA Lifesciences
5-4764-001 		pYSG-IBA 164 N/A N/A N/A
15603828
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IBA Lifesciences
5-4767-001 		pYSG-IBA 167 N/A N/A N/A
15613828
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missing translation for 'viewProductCertificates'
IBA Lifesciences
5-4768-001 		pYSG-IBA 168 N/A N/A N/A
Beschrijving

Beschrijving

pYSG-IBA yeast expression vectors

The pYSG-IBA vector allows the high-level expression of proteins in yeast.

  • The vector carries the copper inducible promoter (CUP1) for controlled high-level expression.
  • The expressed recombinant protein will be localized in the cytoplasm.

 

  • High-level expression in yeast with high copy number
  • Favors correct protein folding and the production of soluble proteins - inclusion bodies rarely form
  • Tightly regulated expression due to Cu++-inducible CUP1 promoter
  • Ampicillin resistance gene and markers Leu2-d and URA3 in every vector
  • Leu2-d auxotrophy marker for selection to increase plasmid copy number during expression
  • URA3 auxotrophy marker for selection after transformation

Please note that cloning into pYSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pYSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specificatie

Specificatie

Liquid
Copper sulfate
8
Acceptor Vector for yeast expression
2°C to 8°C for frequent usage, -20°C for long-term storage
5 μg
Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins
StarGate™ System, Yeast Host Cells
leu2-d and URA3
CUP1
20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
250 ng/μL
Clear
Video's
Veiligheid en behandeling

Veiligheid en behandeling

missing translation for 'shelfLife' : 12 months

SDS
Documentatie

Documentatie

Product Certifications
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