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IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression
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Designed for high-level expression of recombinant proteins in Yeast by introducing the gene of interest under the control of the Cu++-inducible CUP1 promoter.

Merk:  IBA Lifesciences 5-4762-001

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Produktcode. 15683818

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Descripción

Descripción

pYSG-IBA yeast expression vectors

The pYSG-IBA vector allows the high-level expression of proteins in yeast.

  • The vector carries the copper inducible promoter (CUP1) for controlled high-level expression.
  • The expressed recombinant protein will be localized in the cytoplasm.

 

  • High-level expression in yeast with high copy number
  • Favors correct protein folding and the production of soluble proteins - inclusion bodies rarely form
  • Tightly regulated expression due to Cu++-inducible CUP1 promoter
  • Ampicillin resistance gene and markers Leu2-d and URA3 in every vector
  • Leu2-d auxotrophy marker for selection to increase plasmid copy number during expression
  • URA3 auxotrophy marker for selection after transformation

Please note that cloning into pYSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pYSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Especificaciones

Especificaciones

Liquid
Copper sulfate
8
Acceptor Vector for yeast expression
2°C to 8°C for frequent usage, -20°C for long-term storage
250 ng/μL
pYSG-IBA 162
Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins
StarGate™ System, Yeast Host Cells
leu2-d and URA3
CUP1
20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
Twin-Strep-tag for C-terminal and FLAG-tag for N-terminal
5 μg
Clear
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