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  6. Macherey-Nagel™ Protino Ni-NTA Agarose for His-tag Protein Purification

Macherey-Nagel™ Protino Ni-NTA Agarose for His-tag Protein Purification

Method of choice for His-tag protein purification with best performance.

4455.00

Specificatie

Bindingsvermogen <50 mg/mL settled agarose
Beschrijving Aqueous suspension
Content And Storage 4°C to 8°C
Voor gebruik met (toepassing) Purification of His-tag proteins
Doel Protein
Meer specificaties weergeven

Producten 1
Produktcode Merk Aantal Prijs Hoeveelheid en beschikbaarheid  
Produktcode Merk Aantal Prijs Hoeveelheid en beschikbaarheid  
11952192
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Macherey-Nagel™
745400.500 		500 mL
4455.00
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Beschrijving

Beschrijving

  • Enable fast and convenient purification of recombinant polyhistidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
  • Proteins from any expression system can be purified under native or denaturing conditions.
  • Binding of protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions.
  • Consists of the chelating ligand nitrilotriacetic acid (NTA) immobilized on 6% cross-linked agarose beads that are suitable for batch binding, gravity flow, and FPLC™ columns.
  • The resin is precharged with Ni2+ ions and therefore ready to use.
  • Technology: Immobilized metal ion affinity chromatography
  • Batch binding, gravity flow column chromatography, MPLC / FPLC applications
  • Matrix: 6 % beaded agarose (cross-linked), precharged with Ni2+
  • Bead size: 45 to 165 μm
Specificatie

Specificatie

<50 mg/mL settled agarose
4°C to 8°C
Protein
NTA (nitrilotriacetic acid)
Aqueous suspension
Purification of His-tag proteins
Agarose
Video's
Veiligheid en behandeling

Veiligheid en behandeling

Signaalwoord
  • Waarschuwing

Gevarencategorie
  • Ontvlambare vloeistof Categorie 3
  • Sensibilisering van de huid Categorie 1

Gevarenaanduiding
  • H226-Ontvlambare vloeistof en damp.
  • H317-Kan een allergische huidreactie veroorzaken.

Veiligheidsmaatregel
  • P210-Verwijderd houden van warmte, hete oppervlakken, vonken, open vuur en andere ontstekingsbronnen. Niet roken.
  • P261-Inademing van stof/rook/gas/nevel/damp/spuitnevel vermijden.
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.

Aanvullende informatie
  • MIXTURE LIST-Bevat: ethanol (diluted < 20 %) (denatured with1%IPA/1%MEK, acc.2016/1867/EU), resin suspension with Ni in

SDS
Documentatie

Documentatie

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