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Macherey-Nagel™ Protino Ni-NTA Agarose for His-tag Protein Purification
Method of choice for His-tag protein purification with best performance.
Merk: Macherey-Nagel™ 745400.500
This item is not returnable.
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Beschrijving
- Enable fast and convenient purification of recombinant polyhistidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
- Proteins from any expression system can be purified under native or denaturing conditions.
- Binding of protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions.
- Consists of the chelating ligand nitrilotriacetic acid (NTA) immobilized on 6% cross-linked agarose beads that are suitable for batch binding, gravity flow, and FPLC™ columns.
- The resin is precharged with Ni2+ ions and therefore ready to use.
- Technology: Immobilized metal ion affinity chromatography
- Batch binding, gravity flow column chromatography, MPLC / FPLC applications
- Matrix: 6 % beaded agarose (cross-linked), precharged with Ni2+
- Bead size: 45 to 165 μm
Specificatie
| <50 mg/mL settled agarose | |
| 4°C to 8°C | |
| Purification of His-tag proteins | |
| Agarose |
| Aqueous suspension | |
| 500 mL | |
| Protein | |
| NTA (nitrilotriacetic acid) |
Veiligheid en behandeling
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- Waarschuwing
Gevarencategorie
- Ontvlambare vloeistof Categorie 3
- Sensibilisering van de huid Categorie 1
Gevarenaanduiding
- H226-Ontvlambare vloeistof en damp.
- H317-Kan een allergische huidreactie veroorzaken.
Veiligheidsmaatregel
- P210-Verwijderd houden van warmte, hete oppervlakken, vonken, open vuur en andere ontstekingsbronnen. Niet roken.
- P261-Inademing van stof/rook/gas/nevel/damp/spuitnevel vermijden.
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
Aanvullende informatie
- MIXTURE LIST-Bevat: ethanol (diluted < 20 %) (denatured with1%IPA/1%MEK, acc.2016/1867/EU), resin suspension with Ni in
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