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  5. Thermo Scientific Chemicals Phenol:Chloroform:Isoamyl alcohol 25:24:1, Ready-to-Use saturated aq. soln., pH 5.2

Thermo Scientific Chemicals Phenol:Chloroform:Isoamyl alcohol 25:24:1, Ready-to-Use saturated aq. soln., pH 5.2

Phenol-Chloroform-Isoamyl alcohol mixture in the ratio of 25:24:1 (v/v/v), CAS # 136112-00-0, 67-66-3, 123-51-3, is a solution used in the purification of nucleic acids.

131.00 - 401.00

Chemische identificatiekenmerken

CAS 136112-00-0
Moleculaire formule C12H19Cl3O2
Molecular Weight (g/mol) 301.632
MDL-nummer MFCD00133763
InChI Key ZYWFEOZQIUMEGL-UHFFFAOYSA-N
Synoniem phenol-chloroform-isoamyl alcohol mixture, chloroform; isoamyl alcohol; phenol, phenol chloroform isoamyl alcohol, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 125:24:1, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 25:24:1, phenol-chloroform-isoamyl alcohol mixture, vetec tm reagent grade, 25:24:1, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 49.5:49.5:1, phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 5.2, with alkaline buffer, phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 6.7, with alkaline buffer
PubChem CID 66587205
IUPAC Name chloroform;3-methylbutan-1-ol;phenol
SMILES CC(C)CCO.C1=CC=C(C=C1)O.C(Cl)(Cl)Cl
Meer specificaties weergeven

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Produktcode Merk Aantal Prijs Hoeveelheid en beschikbaarheid  
Produktcode Merk Aantal Prijs Hoeveelheid en beschikbaarheid  
15495795
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Thermo Scientific Chemicals
J62336.AE 		100 mL
131.00
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Thermo Scientific Chemicals
J62336.AN 		400 mL
401.00
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Beschrijving

Beschrijving

Phenol:Chloroform:Isoamyl alcohol 25:24:1 is used in the extraction of DNA for the generation of DNA minisatellite fingerprints. It is also used for purifying RNA.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

  • This solution contains a mixture of pure chloroform, isoamyl alcohol and phenol saturated with Tris-EDTA, with a stabilized pH of 8.0

Applications

  • Phenol-Chloroform-Isoamyl alcohol mixture solution (25:24:1) is used in the extraction of nucleic acids, DNA and RNA
  • When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.
  • It is also used to generate DNA minisatellite fingerprints
Specificatie

Chemische identificatiekenmerken

136112-00-0
301.632
ZYWFEOZQIUMEGL-UHFFFAOYSA-N
66587205
CC(C)CCO.C1=CC=C(C=C1)O.C(Cl)(Cl)Cl
C12H19Cl3O2
MFCD00133763
phenol-chloroform-isoamyl alcohol mixture, chloroform; isoamyl alcohol; phenol, phenol chloroform isoamyl alcohol, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 125:24:1, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 25:24:1, phenol-chloroform-isoamyl alcohol mixture, vetec tm reagent grade, 25:24:1, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 49.5:49.5:1, phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 5.2, with alkaline buffer, phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 6.7, with alkaline buffer
chloroform;3-methylbutan-1-ol;phenol

Specificatie

Phenol:Chloroform:Isoamyl alcohol 25:24:1
Ready-to-Use saturated aq. soln.
C12H19Cl3O2
UN2922
Soluble in water.
CC(C)CCO.C1=CC=C(C=C1)O.C(Cl)(Cl)Cl
301.632
5.2
Colorless
136112-00-0
MFCD00133763
phenol-chloroform-isoamyl alcohol mixture, chloroform; isoamyl alcohol; phenol, phenol chloroform isoamyl alcohol, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 125:24:1, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 25:24:1, phenol-chloroform-isoamyl alcohol mixture, vetec tm reagent grade, 25:24:1, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 49.5:49.5:1, phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 5.2, with alkaline buffer, phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 6.7, with alkaline buffer
ZYWFEOZQIUMEGL-UHFFFAOYSA-N
chloroform;3-methylbutan-1-ol;phenol
66587205
Liquid
Video's
Veiligheid en behandeling

Veiligheid en behandeling

GHS H Statement
H331-H314-H341-H351-H373-H302-H312
Toxic if inhaled.
Causes severe skin burns and eye damage.
Suspected of causing genetic defects.
Suspected of causing cancer.
May cause damage to organs through prolonged or repeated exposure.
Harmful if swallowed.
Harmful in contact with skin.

GHS P Statement
P260-P303+P361+P353-P305+P351+P338-P301+P330+P331-P405-P501a
Do not breathe dust/fume/gas/mist/vapors/spray.
IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
Store locked up.
Dispose of contents/container in accordance with local/regional/national/international regulations.

Danger

missing translation for 'dotInformation' : Transport Hazard Class: 8; Packing Group: II; Proper Shipping Name: CORROSIVE LIQUIDS, TOXIC, N.O.S.

missing translation for 'tsca' : No

Recommended Storage : Keep cold

Productidentificatie
  • Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized

Signaalwoord
  • Gevaar

Gevarencategorie
  • Acute toxiciteit Categorie 3
  • Kankerverwekkendheid Categorie 2
  • Ernstig oogletsel/oogirritatie Categorie 1
  • Mutageniteit van geslachtscellen Categorie 2
  • Giftigheid voor de voortplanting Categorie 2
  • Huidcorrosie/-irritatie Indeling Categorie 1B
  • Dzia anie toksyczne na narzady docelowe Categorie 1
  • Dzia anie toksyczne na narzady docelowe Categorie 3

Gevarenaanduiding
  • H301-Giftig bij inslikken.
  • H311-Giftig bij contact met de huid.
  • H314-Veroorzaakt ernstige brandwonden en oogletsel.
  • H318-Veroorzaakt ernstig oogletsel.
  • H331-Giftig bij inademing.
  • H336-Kan slaperigheid of duizeligheid veroorzaken.
  • H341-Verdacht van het veroorzaken van genetische schade.
  • H351-Verdacht van het veroorzaken van kanker.
  • H361d-Wordt ervan verdacht het ongeboren kind te schaden.
  • H372-Veroorzaakt schade aan organen.

Veiligheidsmaatregel
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.
  • P301+P330+P331-NA INSLIKKEN: de mond spoelen - GEEN braken opwekken.
  • P303+P361+P353-BIJ CONTACT MET DE HUID (of het haar): verontreinigde kleding onmiddellijk uittrekken. Huid met water afspoelen/afdouchen.
  • P304+P340-NA INADEMING: de persoon in de frisse lucht brengen en ervoor zorgen dat deze gemakkelijk kan ademen.
  • P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
  • P310-Onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.

Aanvullende informatie
  • MIXTURE LIST-Bevat: Chloroform, Phenol,Isoamyl alcohol

SDS
Documentatie

Documentatie

Product Certifications

RUO – Research Use Only

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