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Thermo Scientific Chemicals Phenol:Chloroform:Isoamyl alcohol 25:24:1, Ready-to-Use saturated aq. soln., pH 5.2
Phenol-Chloroform-Isoamyl alcohol mixture in the ratio of 25:24:1 (v/v/v), CAS # 136112-00-0, 67-66-3, 123-51-3, is a solution used in the purification of nucleic acids.
Merk: Thermo Scientific Chemicals J62336.AE
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| Aantal | 100 mL |
|---|
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Beschrijving
Phenol:Chloroform:Isoamyl alcohol 25:24:1 is used in the extraction of DNA for the generation of DNA minisatellite fingerprints. It is also used for purifying RNA.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
- This solution contains a mixture of pure chloroform, isoamyl alcohol and phenol saturated with Tris-EDTA, with a stabilized pH of 8.0
Applications
- Phenol-Chloroform-Isoamyl alcohol mixture solution (25:24:1) is used in the extraction of nucleic acids, DNA and RNA
- When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.
- It is also used to generate DNA minisatellite fingerprints
Chemische identificatiekenmerken
| 136112-00-0 | |
| 301.632 | |
| ZYWFEOZQIUMEGL-UHFFFAOYSA-N | |
| 66587205 | |
| CC(C)CCO.C1=CC=C(C=C1)O.C(Cl)(Cl)Cl |
| C12H19Cl3O2 | |
| MFCD00133763 | |
| phenol-chloroform-isoamyl alcohol mixture, chloroform; isoamyl alcohol; phenol, phenol chloroform isoamyl alcohol, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 125:24:1, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 25:24:1, phenol-chloroform-isoamyl alcohol mixture, vetec tm reagent grade, 25:24:1, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 49.5:49.5:1, phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 5.2, with alkaline buffer, phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 6.7, with alkaline buffer | |
| chloroform;3-methylbutan-1-ol;phenol |
Specificatie
| Phenol:Chloroform:Isoamyl alcohol 25:24:1 | |
| Ready-to-Use saturated aq. soln. | |
| C12H19Cl3O2 | |
| UN2922 | |
| Soluble in water. | |
| CC(C)CCO.C1=CC=C(C=C1)O.C(Cl)(Cl)Cl | |
| 301.632 | |
| 5.2 | |
| Liquid |
| Colorless | |
| 136112-00-0 | |
| MFCD00133763 | |
| phenol-chloroform-isoamyl alcohol mixture, chloroform; isoamyl alcohol; phenol, phenol chloroform isoamyl alcohol, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 125:24:1, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 25:24:1, phenol-chloroform-isoamyl alcohol mixture, vetec tm reagent grade, 25:24:1, phenol-chloroform-isoamyl alcohol mixture, bioultra, for molecular biology, 49.5:49.5:1, phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 5.2, with alkaline buffer, phenol:chloroform:isoamyl alcohol 25:24:1, ready-to-use saturated aqueous solution, ph 6.7, with alkaline buffer | |
| ZYWFEOZQIUMEGL-UHFFFAOYSA-N | |
| chloroform;3-methylbutan-1-ol;phenol | |
| 66587205 | |
| 100 mL |
Veiligheid en behandeling
Productidentificatie
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized
Signaalwoord
- Gevaar
Gevarencategorie
- Acute toxiciteit Categorie 3
- Kankerverwekkendheid Categorie 2
- Ernstig oogletsel/oogirritatie Categorie 1
- Mutageniteit van geslachtscellen Categorie 2
- Giftigheid voor de voortplanting Categorie 2
- Huidcorrosie/-irritatie Indeling Categorie 1B
- Dzia anie toksyczne na narzady docelowe Categorie 1
- Dzia anie toksyczne na narzady docelowe Categorie 3
Gevarenaanduiding
- H301-Giftig bij inslikken.
- H311-Giftig bij contact met de huid.
- H314-Veroorzaakt ernstige brandwonden en oogletsel.
- H318-Veroorzaakt ernstig oogletsel.
- H331-Giftig bij inademing.
- H336-Kan slaperigheid of duizeligheid veroorzaken.
- H341-Verdacht van het veroorzaken van genetische schade.
- H351-Verdacht van het veroorzaken van kanker.
- H361d-Wordt ervan verdacht het ongeboren kind te schaden.
- H372-Veroorzaakt schade aan organen.
Veiligheidsmaatregel
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
- P301+P330+P331-NA INSLIKKEN: de mond spoelen - GEEN braken opwekken.
- P303+P361+P353-BIJ CONTACT MET DE HUID (of het haar): verontreinigde kleding onmiddellijk uittrekken. Huid met water afspoelen/afdouchen.
- P304+P340-NA INADEMING: de persoon in de frisse lucht brengen en ervoor zorgen dat deze gemakkelijk kan ademen.
- P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
- P310-Onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
Aanvullende informatie
- MIXTURE LIST-Bevat: Chloroform, Phenol,Isoamyl alcohol
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