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Macherey-Nagel™ NucleoSpin™ RNA Plant, Mini kit for RNA from plant
Isolates total RNA from all kinds of plant material and filamentous fungi without degradation of the RNA. Macherey-Nagel™ NucleoSpin™ RNA Plant Kit is a complete mini spin kit that includes RNA plant columns with collection tubes, filters, all needed buffers and RNase-free rDNase.
Merk: Macherey-Nagel™ 740949.50
Beschrijving
NucleoSpin™ RNA Plant Kit is designed for isolation of DNA-free, high-quality RNA from a wide variety of plant and fungal samples. Typical downstream applications include real-time RT-PCR, gene expression profiling, Northern blotting, primer extension, array technology, and RNase protection assays.
- Two different lysis buffers included—Optimized lysis procedure for typical conditions and for peculiar metabolites
- rDNase included for on-column digestion—Efficient removal of contaminating DNA
- NucleoSpin™ Filters (shredders) included—Efficient sample homogenization and reduction of viscosity
- Parallel purification of DNA possible—By using the NucleoSpin™ RNA/DNA buffer set
Kit Components: NucleoSpin™ RNA columns with 2 mL collection tubes, 2 mL and 1.5 mL collecting tubes, NucleoSpin™ filters, buffers (lysis, wash, desalting), RNase-free DNase I, reaction buffer for DNase, RNase-free water
- Mini spin column with silica membrane technology
- Yields 3 to 70μg RNA from 100μg plant material
- Elution volume: 40 to 60μL
- Fragment size: 200b–20kb
- Binding capacity: 200μg
- Preparation time: 30 min./6 preps
- A260/A280: 1.9–2.1
Specificatie
Mini Spin Column | |
RNA purification from plant cells and tissue | |
50 Preps | |
1 Each | |
3–70μg |
Real-time RT-PCR | |
Lysis buffer RA1 (25mL), Lysis buffer RAP (25mL), Wash buffer RAW2 (13mL), Wash Buffer RA3 concentrate (12mL), Membrane desalting buffer (25mL), Reaction buffer for rDNase (7mL), RNase-free rDNase (1 size F vial), RNase-free water (13mL), NucleoSpin filters (50), NucleoSpin RNA Plan Columns with collection tubes (50), 2mL Collection tubes (150), 1.5mL Collection tubes (50) | |
NucleoSpin™ RNA Plant Column | |
<100 mg |
Veiligheid en behandeling
- Gevaar
Gevarencategorie
- Ontvlambare vloeistof Categorie 3
- Acute toxiciteit Categorie 4
- Huidcorrosie/-irritatie Indeling Categorie 2
- Ernstig oogletsel/oogirritatie Categorie 2
- Sensibilisatie van de luchtwegen Categorie 1
- AQUATISCH GEVAAR OP LANGE TERMIJN Categorie 3 voor chronische toxiciteit
Gevarenaanduiding
- H226-Ontvlambare vloeistof en damp.
- H302-Schadelijk bij inslikken.
- H315-Veroorzaakt huidirritatie.
- H319-Veroorzaakt ernstige oogirritatie.
- H334-Kan bij inademing allergie- of astmasymptomen of ademhalingsmoeilijkheden veroorzaken.
- H412-Schadelijk voor in het water levende organismen, met langdurige gevolgen.
Veiligheidsmaatregel
- P261-Inademing van stof/rook/gas/nevel/damp/spuitnevel vermijden.
- P342+P311-Bij ademhalingssymptomen: Een ANTIGIFCENTRUM/arts/ raadplegen.
Aanvullende informatie
- MIXTURE LIST-Bevat: ethanol (diluted < 20 %)(denatured with1%IPA/1%MEK, acc.2016/1867/EU); guanidinium thiocyanate; gua
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