Cell Culture Media
Gibco™ Essential 8™ Medium
Essential 8™ Medium
Gibco™ RPMI 1640 Medium (ATCC modification)
Originally developed to culture human leukemic cells in suspension and as a monolayer
Gibco™ Cell Dissociation Buffer, enzyme-free, PBS
Animal origin-free
Gibco™ DMEM, no glucose
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, No Glucose is used in cell culture processing applications.
Gibco™ N-2 Supplement (100X)
N-2 Supplement (100X)
Gibco™ Advanced DMEM/F-12
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ Advanced DMEMF/12 is used in cell culture processing applications.
Gibco™ Recovery™ Cell Culture Freezing Medium
Complete, ready-to-use freezing medium for cryopreservation of a wide variety of mammalian cells, including CHO-S, CHO-K1, HEK 293, Jurkat, and NIH 3T3
Gibco™ Opti-MEM™ I Reduced Serum Medium
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.
Gibco™ Essential 8™ Flex Medium Kit
Essential 8™ Flex is a xeno-free and feeder-free medium that supports the culture of pluripotent stem cells (PSCs) without the need for weekend feeding. Gibco™ Essential 8™ Flex Medium Kit is provided in a convenient two-component kit (500mL basal medium and 10mL supplement).
Gibco™ MEM
Minimum Essential Medium is one of the most commonly used of all cell culture media
Gibco™ DMEM/F-12, GlutaMAX™ supplement
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEMF/12, GlutaMAX™ Supplement is used in cell culture processing applications.
Gibco™ IMDM, no phenol red
Suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells
Gibco™ RPMI 1640 Medium, no glucose
Originally developed to culture human leukemic cells in suspension and as a monolayer
Gibco™ Opti-MEM™ I Reduced Serum Medium, no phenol red
Improved Minimal Essential Medium that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in cell growth rate or morphology
Gibco™ DMEM, no glucose, no glutamine, no phenol red
Supports growth of various mammalian cells
Gibco™ DMEM, high glucose, no glutamine
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, No Glutamine is used in cell culture processing applications.
Gibco™ DMEM/F-12
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM/F-12 is used in cell culture processing applications.
Gibco™ Ham's F-12 Nutrient Mix
Designed for serum-free, single-cell plating of Chinese hamster ovary (CHO) cells
Gibco™ IMDM
Iscove's Modified Dulbecco's Medium is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells
Gibco™ DMEM, high glucose, pyruvate
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, Pyruvate is used in cell culture processing applications.
Gibco™ Ham's F-12K (Kaighn's) Medium
A modification of Ham's F-12 Nutrient Mixture
Gibco™ Essential 6™ Medium
Essential 6™ Medium
Gibco™ DMEM, high glucose
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose is used in cell culture processing applications.
Gibco™ RPMI 1640 Medium, HEPES
Originally developed to culture human leukemic cells in suspension and as monolayer
Gibco™ MEM α, nucleosides, no ascorbic acid
Minimum Essential Medium is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells
Gibco™ MEM α, GlutaMAX™ Supplement, no nucleosides
Minimum Essential Medium is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells
Gibco™ DMEM, high glucose, HEPES, no phenol red
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, HEPES, No Phenol Red is used in cell culture processing applications.
Gibco™ DMEM/F-12, no phenol red
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEMF/12, No Phenol Red is used in cell culture processing applications.
Cell culture media support the growth and maintenance of a variety of mammalian and other cell lines in vitro, as either anchorage-dependent or suspension cultures. Cell culture offers convenience, control, consistency and reproducibility of results, especially when using clonal cells.
Cell culture has multiple applications in cellular and molecular biology:
- Studying normal cell physiology and biochemistry
- Industrial bioprocess/bioreactor manufacturing of vaccines, therapeutic proteins and monoclonal antibodies using recombinant, high-yield protein expression systems
- Pharmaceutical screening and development, including drug metabolism and toxicology
- Other emerging applications in stem cell culture, immunotherapy and cell therapy
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
- Classical mammalian cell culture media (DMEM, MEM, RPMI 1640, McCoy’s, Ham’s, BME and EBSS) contain all the nutrients that cells need, such as amino acids, minerals, vitamins and glucose
- Individual cell culture reagents and supplements, including growth factors (e.g. pyruvate), antibiotics, hormones, cryopreservation media, cell dissociation reagents and animal-derived sera (e.g., FBS foetal bovine serum) which contain a ready-made natural mixture of nutrients
- Speciality media, including stem cell culture media, transfection media, and serum-free or animal-derived component-free (ACDF) additives
- Ancillary buffers and process liquids, including balanced salt solutions (PBS phosphate buffered saline, Hank’s HBSS, Earle’s EBBS), sodium bicarbonate and HEPES buffers, phenol red, cell culture grade water, and adherent cell microcarriers
Cell culture media are typically supplied as presterilised ready-to-use liquids, concentrates, powders or other solids in bottles, or else as single-use sealed bags for integration into compatible cell culture harvesting systems.
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories: