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IBA Lifesciences Strep-Tactin™ Magnetic Microbeads
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IBA Lifesciences GmbH Strep-Tactin™ Magnetic Microbeads enable a rapid and economic cell isolation.

Merk:  IBA Lifesciences 6-5510-050

Produktcode. 16441485

  • 290.00 /

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Beschrijving

Beschrijving

  • Strep-Tactin™ Magnetic Microbeads are microspheres covalently linked to Strep-Tactin™.
  • They are suitable for labeling antigen-specific cytotoxic T cells (MHC I Streptamer™) or surface marker-specific cells (Fab-TACS™/Nano-TACS™).
  • To target specific cells, MHC I complexes, low affinity Fab fragments or nanobodies fused to a Twin-Strep-tag™ (MHC I-Streps, Fab-Streps or Nano-Streps) are required in addition to fluorescent Strep-Tactin™.

  • Strep-Tactin™ multimerizes MHC I-Streps or Fab-/Nano-Streps, ensuring stable binding to cells.
  • The resulting magnetic complexes can be used to label cells that are specific for the respective peptide-loaded MHC I molecule or for a selected surface marker.
  • The addition of biotin reverses the multimerization and causes the dissociation of MHC I-Streps or Fab-/Nano-Streps from the cell surface.
  • The complete removal of all labeling reagents permits serial positive selection as well as depletion steps and reserves the cells’ full biological activity.
Specificatie

Specificatie

750 μL
Suspension in Dulbeccos phosphate buffered saline (DPBS) without Ca and Mg, contains KCl and 0.1% sodium azide
1 to 3 μm
Magnetic Affinity Bead
Strep-Tactin™ covalently linked to polysaccharide coated magnetic microbeads
2°C to 8°C, do not freeze
750 μL for isolation out of 5 x 108 cells
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