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IBA Lifesciences StarGate pPSG-IBA Acceptor Vectors for E. coli Expression

Achieves high-level expression in E. coli by the T7 promoter by providing specified genetic surroundings for optimal expression and purification of non-toxic proteins.

Merk:  IBA Lifesciences 5-4233-001

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Produktcode. 15683758

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Beschrijving

Beschrijving

pPSG-IGA E. coli expression vectors:

pPSG-IBA vectors use the T7 promoter and T7 RNA polymerase for high-level transcription of the gene of interest.

  • As the T7 promoter is stronger than the tet promoter, pPSG-IBA vectors are recommended in cases where expression with the tet promoter does not lead to significant yields of the recombinant protein.

    • High-level expression in E. coli by bacteriophage T7 promoter
    • High-level transcription by T7 RNA polymerase in BL21 strains
    • High-level expression of non-toxic proteins
    • Induction by IPTG
    • Suitable for in vitro transcription/translation

    StarGate System

    IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:

    • Minimal extra modification of the gene due to short combinatorial sites
    • Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
    • GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
    • Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
    • The final expression vector, the Destination Vector, is then placed into the respective host
    • Specificatie

    Specificatie

    Liquid
    E. coli host with T7 RNA polymerase gene (e.g. BL21(DE3))
    T7
    20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
    His Tag for C-terminal
    5 μg
    Clear
    StarGate™ System, E. Coli BL21(DE3)
    8
    Acceptor Vector for E. coli expression
    2°C to 8°C for frequent usage, -20°C for long-term storage
    250 ng/μL
    pPSG-IBA 33
    Cloning genes of interest for use in functional expression, purification, detection, immobilization or separation of recombinant proteins
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