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Cytiva Sera-Xtracta Cell-Free DNA Kit
Sera-Xtracta Cell-Free DNA Kit for the efficient extraction and purification of cell-free DNA (cfDNA) from plasma.
Merk: Cytiva 29437807
This item is not returnable.
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Beschrijving
Designed to select for small-fragment cfDNA while minimizing genomic DNA contamination, Sera-Xtracta Cell-Free DNA Kit offers efficient extraction and purification of cfDNA from plasma. High yield and sensitivity make the kit well-suited for applications such as cancer diagnosis and monitoring where sample is precious.
- Efficient recovery of small-fragment cfDNA in the size range 50-300 bp: capture more cfDNA to increase sensitivity levels.
- Minimal co-purification of higher molecular weight genomic DNA due to active selection of smaller fragments which improves sensitivity in downstream applications.
- Scalability: one kit to cover 0.5 mL to 4 mL input of plasma.
- Efficient extraction: procedure can be completed in less than 120 minutes.
- Ease of use: compatible with molecular biology techniques, including next-generation sequencing (NGS), qPCR, ddPCR, BEAMing and other amplification and genotyping applications.
The method uses chaotropic agents and detergents to disrupt protein components to which the cfDNA may initially bind and to promote the selective binding of this cfDNA to the silica magnetic beads.
Proteinase K is used to digest protein from samples; denatured contaminants are easily removed by subsequent washing of the silica beads with specially formulated wash buffers. Proteinase K treatment is also required to release the cfDNA from plasma derived from blood collected in tubes with cfDNA stabilization, such as Cell Free DNA BCT™ (Streck™) for reversal of the stabilization/release of cfDNA.
The purified cfDNA is eluted in a low ionic strength buffer, at a concentration suitable for most downstream molecular biology applications, such as next-generation sequencing (NGS).
Specificatie
| Proteinase K 2 x 60 mg (dried); SDS 20% 12 mL; Magnetic silica bead suspension 1.6 mL; Binding buffer 2 x 150 mL; Wash buffer 1-AQ 85 mL; Wash buffer 2-AQ 35 mL; cfDNA Elution buffer: 10 mL. All kit components should be stored at room temperature (15 to 30°C). For Proteinase K, once reconstituted, store at 2 to 8°C | |
| 30 μL | |
| 96 Purifications | |
| Minimal higher molecular weight genomic DNA contamination due to active selection of smaller fragments |
Veiligheid en behandeling
Productidentificatie
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- part of Sera-Xtracta Cell-Free DNA kit
Signaalwoord
- Gevaar
Gevarencategorie
- Acute toxiciteit Categorie 4
- AQUATISCH GEVAAR OP LANGE TERMIJN Categorie 3 voor chronische toxiciteit
- Ernstig oogletsel/oogirritatie Categorie 1
- Ernstig oogletsel/oogirritatie Categorie 2
- Sensibilisatie van de luchtwegen Categorie 1
- Huidcorrosie/-irritatie Indeling Categorie 2
- Dzia anie toksyczne na narzady docelowe Categorie 3
Gevarenaanduiding
- H302-Schadelijk bij inslikken.
- H315-Veroorzaakt huidirritatie.
- H318-Veroorzaakt ernstig oogletsel.
- H319-Veroorzaakt ernstige oogirritatie.
- H334-Kan bij inademing allergie- of astmasymptomen of ademhalingsmoeilijkheden veroorzaken.
- H335-Kan irritatie van de luchtwegen veroorzaken.
- H412-Schadelijk voor in het water levende organismen, met langdurige gevolgen.
Veiligheidsmaatregel
- P260-Stof/rook/gas/nevel/damp/spuitnevel niet inademen.
- P304+P340-NA INADEMING: de persoon in de frisse lucht brengen en ervoor zorgen dat deze gemakkelijk kan ademen.
- P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
- P310-Onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
- P405-Achter slot bewaren.
- P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.
Aanvullende informatie
- MIXTURE LIST-Bevat: proteases, sodium dodecyl sulphate, salts of thiocyanic acid
- UNKNOWN ACUTE-Het mengsel bestaat voor 22.5 procent uit een of meer bestanddelen waarvan de acute toxiciteit niet bekend is.
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