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Thermo Scientific™ Pierce™ Protein Refolding Kit
Refold recombinant proteins that have been denatured and solubilized from inclusion bodies by determining optimal buffer conditions using this reagent matrix strategy.
Merk: Thermo Scientific™ 89867
572.36 EUR valid until 2025-06-30
Use promo code "24108" to get your promotional price.
Waarschuwingen:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price
Beschrijving
Thermo Scientific™ Pierce Protein Refolding Kit includes high-purity reagents and detailed instructions for using a matrix strategy to determine optimal buffer conditions for refolding recombinant proteins that have been denatured and solubilized from inclusion bodies.
The kit contains the essential reagents and complete strategy for determining optimal buffer conditions to refold denatured recombinant proteins to restore native structure and function. Nine base refolding buffers form a matrix that includes a range of strong and weak denaturant conditions for the suppression of protein aggregation. The supplied additives are used as additional matrix factors, depending on the protein type being refolded. Buffer components are examined at three concentration levels, allowing a wide spectrum of folding conditions to be tested within one experiment. The adjustable design allows matrix conditions to be tailored to the target protein, preventing sample waste and unnecessary analysis, while maximizing refolding yields. The Pierce Protein Refolding Kit is accompanied by a comprehensive Refolding Guide with details on isolating, solubilizing and purifying inclusion bodies; optimizing refolding conditions; and analyzing refolding yields.Highlights:
Robust – conditions and components examined are limited to those having the most significant and general utility as folding buffers
Convenient – three-level matrix design significantly reduces the amount of secondary optimization required and increases the ease of data interpretation
Adjustable matrix format – allows refolding experiments to be customized to the target protein; known positive and negative interactions between buffer components are addressed, minimizing unnecessary analyses
High-purity reagents – reagents are formulated using stringent standards so that consistent results are attained
Includes:
Nine different Base Refolding Buffers (10mL each), convenient amounts of seven additives, and a comprehensive Refolding Guidebook
Compatible with:
Thermo Scientific Inclusion Body Solubilization Reagent (Part No. 78115)
Specificatie
| Protein Refolding Kit | |
| Liquid, Powder | |
| Chaotrope, Denaturant |
| Upon receipt store at -20°C. Product may be stored at 4°C for up to two months. Product is shipped on dry ice. | |
| Pierce™ | |
| 100 Reactions |
Veiligheid en behandeling
Productidentificatie
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- Pierce Protein Refolding Kit
Signaalwoord
- Waarschuwing
Gevarencategorie
- Acute toxiciteit Categorie 4
- AQUATISCH GEVAAR OP LANGE TERMIJN Categorie 3 voor chronische toxiciteit
- Ernstig oogletsel/oogirritatie Categorie 2
- Huidcorrosie/-irritatie Indeling Categorie 2
- Dzia anie toksyczne na narzady docelowe Categorie 3
Gevarenaanduiding
- H302-Schadelijk bij inslikken.
- H315-Veroorzaakt huidirritatie.
- H319-Veroorzaakt ernstige oogirritatie.
- H335-Kan irritatie van de luchtwegen veroorzaken.
- H412-Schadelijk voor in het water levende organismen, met langdurige gevolgen.
Veiligheidsmaatregel
- P261-Inademing van stof/rook/gas/nevel/damp/spuitnevel vermijden.
- P264-Na het werken met dit product grondig wassen.
- P270-Niet eten, drinken of roken tijdens het gebruik van dit product.
- P271-Alleen buiten of in een goed geventileerde ruimte gebruiken.
- P273-Voorkom lozing in het milieu.
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
- P301+P312-NA INSLIKKEN: bij onwel voelen een ANTIGIFCENTRUM / arts/ raadplegen.
- P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
- P304+P340-NA INADEMING: de persoon in de frisse lucht brengen en ervoor zorgen dat deze gemakkelijk kan ademen.
- P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
- P312-Bij onwel voelen een ANTIGIFCENTRUM/arts/ raadplegen.
- P330-De mond spoelen.
- P332+P313-Bij huidirritatie: een arts raadplegen.
- P337+P313-Bij aanhoudende oogirritatie: een arts raadplegen.
- P362+P364-Verontreinigde kleding uittrekken en wassen alvorens deze opnieuw te gebruiken.
- P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.
Aanvullende informatie
- MIXTURE LIST-Bevat: (R*,R*)-1,4-dimercapto-2,3-butanediol, Sodium chloride
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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