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Thermo Scientific™ HABA (4'-hydroxyazobenzene-2-carboxylic acid)
A colored, biotin assay reagent HABA (4'-hydroxyazobenzene-2-carboxylic acid) to measure biotinylation levels of biotinylated antibodies or proteins.
Merk: Thermo Scientific™ 28010
162.45 EUR valid until 2025-05-31
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Beschrijving
Thermo Scientific™ Pierce HABA is 4'-hydroxyazobenzene-2-carboxylic acid, a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation levels of labeled proteins and other molecules.
Thermo Scientific Pierce HABA is 4'-hydroxyazobenzene-2-carboxylic acid, a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation levels of labeled proteins and other molecules. Determine the molar ratio of biotin incorporated into a protein using the HABA-Avidin method. The HABA dye (4'-hydroxyazobenzene-2-carboxylic acid ) binds to avidin to produce a yellow-orange colored complex which absorbs at 500nm. Free biotin will displace the HABA dye and cause the absorbance to decrease. A standard curve can be established using the free biotin to estimate the number of moles of biotin incorporated after biotinylating a protein.
Highlights:
- HABA-avidin complex can be used over a wide range of pH and salt concentrations
- Amount of Avidin can be calculated directly from the increased absorbance at 500nm complexing with the HABA Dye
- Calculate results directly from absorbance values based on extinction coefficients using the procedure outlined in the instructions or use Pierce's online HABA calculator
- Complete kits also available! See Pierce Biotin Quantitation Kit (Part No. 28005) and Fluorescence Biotin Quantitation Kit (Part No. 46610)
Specificatie
| HABA | |
| Upon receipt store product at room temperature. Product shipped at ambient temperature. | |
| Biotin |
| 10 g | |
| Pierce™ | |
| Antibodies, Proteins |
Veiligheid en behandeling
Productidentificatie
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- HABA (4'-hydroxyazobenzene-2-carboxylic acid)
Signaalwoord
- Waarschuwing
Gevarencategorie
- Ernstig oogletsel/oogirritatie Categorie 2
- Huidcorrosie/-irritatie Indeling Categorie 2
- Dzia anie toksyczne na narzady docelowe Categorie 3
Gevarenaanduiding
- H315-Veroorzaakt huidirritatie.
- H319-Veroorzaakt ernstige oogirritatie.
- H335-Kan irritatie van de luchtwegen veroorzaken.
Veiligheidsmaatregel
- P260-Stof/rook/gas/nevel/damp/spuitnevel niet inademen.
- P264-Na het werken met dit product grondig wassen.
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
- P301+P330+P331-NA INSLIKKEN: de mond spoelen - GEEN braken opwekken.
- P303+P361+P353-BIJ CONTACT MET DE HUID (of het haar): verontreinigde kleding onmiddellijk uittrekken. Huid met water afspoelen/afdouchen.
- P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
- P405-Achter slot bewaren.
- P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.
Aanvullende informatie
- MIXTURE LIST-Bevat: 2-(p-hydroxyphenylazo)benzoic acid
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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