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Thermo Scientific™ Pierce™ kleuringset voor glycoproteïne
Rapidly stain glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method in this convenient kit.
Merk: Thermo Scientific™ 24562
383.80 EUR valid until 2025-05-31
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Beschrijving
Thermo Scientific™ Pierce Glycoprotein Staining Kit is a convenient, fast and sensitive kit for staining glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method.
The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins (glycoproteins) that have been separated by polyacrylamide gel electrophoresis (PAGE). A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions. The kit includes positive and negative control proteins.Highlights:
Specific for glycoproteins – periodic acid-Schiff (PAS) method specifically detects glycosylated proteins having sialic acid and other oxidizable carbohydrate groups
Optimized protocol – the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours (half the time required for other staining methods)
Colorimetric – glycoproteins are detected as magenta bands against a faint pink or colorless gel background
Qualitative staining – detection limit depends on the extent of glycosylation and protein size and abundance; detects 0.625ng avidin and 0.16g horseradish peroxidase per band
Controls included – complete kit includes positive and negative control proteins (HRP and STI, respectively).
Gels and membranes – effective for use with polyacrylamide mini gels or nitrocellulose membranes
Includes:
Three reagents in 250mL bottles (1 solution and 2 dry powders to which water must be added), plus two vials of lyophilized proteins (1mg each)
Specificatie
| Sufficient For: 10 mini gels (SDS-PAGE) • Oxidation Reagent, 2.5 g powder to make 250 mL • Reduction Reagent, 1.25 g powder to make 250 mL • Positive Control (Horseradish Peroxidase), 1 mg • Negative Control (Soybean Trypsin Inhibitor), 1 mg • Glycoprotein Stain, 250 mL Store all components at 4°C. |
|
| Colorimetric | |
| Glycoprotein Staining Kit | |
| 750 mL |
| In-Blot Detection, In-Gel Detection | |
| Fuchsin Sulfite | |
| Pierce™ | |
| Proteins (Glycoproteins) |
Veiligheid en behandeling
Productidentificatie
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
- Pierce Glycoprotein Staining Kit
Signaalwoord
- Gevaar
Gevarencategorie
- Oxiderende vaste stof Categorie 2
- Acute toxiciteit Categorie 4
- Huidcorrosie/-irritatie Indeling Categorie 1B
- Ernstig oogletsel/oogirritatie Categorie 1
- Sensibilisering van de huid Categorie 1
- Sensibilisatie van de luchtwegen Categorie 1
- Kankerverwekkendheid Categorie 1B
Gevarenaanduiding
- H272-Kan brand bevorderen; oxiderend.
- H302-Schadelijk bij inslikken.
- H314-Veroorzaakt ernstige brandwonden en oogletsel.
- H317-Kan een allergische huidreactie veroorzaken.
- H334-Kan bij inademing allergie- of astmasymptomen of ademhalingsmoeilijkheden veroorzaken.
- H350-Kan kanker veroorzaken.
- EUH031-Vormt giftig gas in contact met zuren.
Veiligheidsmaatregel
- P201-Alvorens te gebruiken de speciale aanwijzingen raadplegen.
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
- P284-¢Bij ontoereikende ventilatie! adembescherming dragen.
- P210-Verwijderd houden van warmte, hete oppervlakken, vonken, open vuur en andere ontstekingsbronnen. Niet roken.
- P220-Van kleding/brandbare stoffen verwijderd houden/bewaren.
- P260-Stof/rook/gas/nevel/damp/spuitnevel niet inademen.
- P264-Na het werken met dit product grondig wassen.
- P270-Niet eten, drinken of roken tijdens het gebruik van dit product.
- P301+P310-NA INSLIKKEN: onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
- P331-GEEN braken opwekken.
- P303+P361+P353-BIJ CONTACT MET DE HUID (of het haar): verontreinigde kleding onmiddellijk uittrekken. Huid met water afspoelen/afdouchen.
- P304+P340-NA INADEMING: de persoon in de frisse lucht brengen en ervoor zorgen dat deze gemakkelijk kan ademen.
- P305-BIJ CONTACT MET DE OGEN:.
- P310-Onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
- P501-Inhoud/verpakking afvoeren naar .
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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