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Thermo Scientific™ Pierce™ DTNB (Ellman's reagens)

Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).

Merk:  Thermo Scientific™ 22582

122.55 EUR valid until 2025-05-31
Use promo code "25977" to get your promotional price.


Produktcode. 10086113

  • 133.00 /

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Beschrijving

Beschrijving

Thermo Scientific™ Pierce DTNB (Ellman's Reagent) reacts with sulfhydryl groups to yield a colored product, which provides a reliable method for measure free reduced cysteines and other free sulfhydryls in solution.

A solution of this compound produces a measurable yellow-colored product when it reacts with sulfhydryls. Consequently, Ellman's Reagent is very useful as a sulfhydryl assay reagent because of its specificity for –SH groups at neutral pH, high molar extinction coefficient and short reaction time. Sulfhydryl groups may be estimated in a sample by comparison to a standard curve composed of known concentrations of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups may be quantitated by reference to the extinction coefficient of TNB.

Highlights:

  • Sulfhydryl detection– reacts quantitatively with free (reduced) sulfhydryl groups

  • Colorimetric–yields colored product to enable spectrophotemetric measurement in tubes or microplates

  • Proven assay method– well-characterized chemistry enables quantitation of peptide or protein sulfhydryl groups using extinction coefficient calculation or by comparison to a cysteine standard

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

DTNB
5 g
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Veiligheid en behandeling

Veiligheid en behandeling

Productidentificatie
  • DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)

Signaalwoord
  • Waarschuwing

Gevarencategorie
  • Ernstig oogletsel/oogirritatie Categorie 2
  • Huidcorrosie/-irritatie Indeling Categorie 2
  • Dzia anie toksyczne na narzady docelowe Categorie 3

Gevarenaanduiding
  • H315-Veroorzaakt huidirritatie.
  • H319-Veroorzaakt ernstige oogirritatie.
  • H335-Kan irritatie van de luchtwegen veroorzaken.

Veiligheidsmaatregel
  • P260-Stof/rook/gas/nevel/damp/spuitnevel niet inademen.
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.
  • P304+P340-NA INADEMING: de persoon in de frisse lucht brengen en ervoor zorgen dat deze gemakkelijk kan ademen.
  • P310-Onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
  • P405-Achter slot bewaren.
  • P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.

Aanvullende informatie
  • MIXTURE LIST-Bevat: 3,3'-dithiobis [6-nitrobenzoic] acid

SDS
Documentatie

Documentatie

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For Research Use Only. Not for use in diagnostic procedures.