Thermo Scientific™ Pierce™ DSP-crosslinker

Beschrijving

Thermo Scientific™ Pierce DSP Crosslinker (Lomant's Reagent) is an amine-reactive crosslinker with NHS-ester reactive ends and a cleavable disulfide bond in its spacer arm.

Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging helps prevent loss of reagent reactivity and contamination over time by minimizing repetitive opening and closing of vial. The format enables use of fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.

• Reactive groups: NHS ester (both ends)
• Reactive towards: amino groups (primary amines)
• Amine-reactive NHS ester reacts rapidly with any primary amine-containing molecule
• Disulfide bond in the spacer arm is readily cleaved by 10-50mM DTT or TCEP at pH 8.5
• Spacer arm also cleavable with reducing SDS-PAGE sample loading buffer
• Cleavable crosslinker allows separation of crosslinked products
• Water-insoluble (dissolve first in DMF or DMSO); compare to DTSSP (Sulfo-DSP)
• Membrane-permeable, allowing for intracellular crosslinking

• Chemical crosslinking of intracellular proteins prior to cell lysis and immunoprecipitation
• "Fix" protein interactions to allow identification of weak or transient protein interactions
• Fix tissues for immunostaining without damaging RNA
• Protein crosslinking to create bioconjugates via single-step reactions
• Immobilize proteins onto amine-coated surfaces
• Examining spatial relationships of the capside polypeptides of the mengo virion
• Studying renal Na+ and K+ -ATPase
• Nearest neighbor relationships of bovine mitochondrial H+ -ATP
• Producing interactions between protein components of the chemotaxis mechanism in E. coli
• Chemical crosslinking of a-CPI