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Thermo Scientific™ Pierce™ isolatiekit voor celoppervlakeiwit

Selectively biotinylate and purify mammalian cell surface proteins and exclude intracellular proteins with this complete set of reagents.

Merk:  Thermo Scientific™ 89881

506.69 EUR valid until 2025-06-27
Use promo code "24112" to get your promotional price.


Produktcode. 10230104

  • 760.00 /

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Waarschuwingen:

To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price

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Beschrijving

Beschrijving

Thermo Scientific™ Cell Surface Protein Isolation Kit is a complete set of reagents for selective biotinylation and subsequent purification of mammalian cell surface proteins to the exclusion of intracellular proteins.

The kit efficiently labels proteins that have accessible lysine residues and sufficient extracellular exposure.The isolation procedure uses a cell-impermeable, cleavable biotinylation reagent (Sulfo-NHS-SS-Biotin) to label exposed primary amines of proteins on the surface of intact adherent or suspension cells. Treated cells are then harvested and lysed, and the labeled surface proteins are affinity-purified using Thermo Scientific NeutrAvidin Agarose Resin. The isolated cell surface proteins contain a small, nonreactive tag of the originally labeled primary amines but are no longer biotinylated (biotin remains bound to the resin). The kit contains sufficient reagents for eight experiments, each involving four T75 flasks of confluent cells.

Highlights:

  • Isolates cell surface proteins – reduces complexity of total cellular protein

  • Efficiently recovers labeled proteins – cleavable biotin allows for nearly 100% recovery of isolated cell surface proteins

  • Convenience – all reagents are provided in one kit, along with complete instructions for labeling, cell lysis and purification of cell surface membrane proteins

  • Western blotting applications – proteins recovered in SDS-PAGE buffer are loaded directly onto polyacrylamide gels

  • Robust system – protocol designed for diverse cell lines, including NIH 3T3, HeLa, C6 and A431

Includes:

Biotinylation reagent, cell lysis reagent, agarose affinity resin, various buffers and microcentrifuge spin columns

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

Protein Isolation Kit
Liquid, Powder
Lysis Buffer
8 Reactions
Upon receipt store kit at 4°C. Product shipped at ambient temperature.
Pierce™
Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation
8 reactions kit
Product Suggestions

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Video's
Veiligheid en behandeling

Veiligheid en behandeling

Productidentificatie
  • CELL SURFACE PROT ISOLATION KIT

Signaalwoord
  • Waarschuwing

Gevarencategorie
  • Ernstig oogletsel/oogirritatie Categorie 2
  • Huidcorrosie/-irritatie Indeling Categorie 2
  • Dzia anie toksyczne na narzady docelowe Categorie 3
  • Acute toxiciteit Categorie 4
  • AQUATISCH GEVAAR OP LANGE TERMIJN Categorie 3 voor chronische toxiciteit

Gevarenaanduiding
  • H319-Veroorzaakt ernstige oogirritatie.
  • H302-Schadelijk bij inslikken.
  • H315-Veroorzaakt huidirritatie.
  • H335-Kan irritatie van de luchtwegen veroorzaken.
  • H412-Schadelijk voor in het water levende organismen, met langdurige gevolgen.

Veiligheidsmaatregel
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.
  • P264-Na het werken met dit product grondig wassen.
  • P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
  • P261-Inademing van stof/rook/gas/nevel/damp/spuitnevel vermijden.
  • P270-Niet eten, drinken of roken tijdens het gebruik van dit product.
  • P273-Voorkom lozing in het milieu.
  • P304+P340-NA INADEMING: de persoon in de frisse lucht brengen en ervoor zorgen dat deze gemakkelijk kan ademen.
  • P312-Bij onwel voelen een ANTIGIFCENTRUM/arts/ raadplegen.
  • P301+P312-NA INSLIKKEN: bij onwel voelen een ANTIGIFCENTRUM / arts/ raadplegen.
  • P330-De mond spoelen.
  • P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
  • P362+P364-Verontreinigde kleding uittrekken en wassen alvorens deze opnieuw te gebruiken.
  • P332+P313-Bij huidirritatie: een arts raadplegen.
  • P337+P313-Bij aanhoudende oogirritatie: een arts raadplegen.

Aanvullende informatie
  • MIXTURE LIST-Bevat: Sodium chloride

SDS
Documentatie

Documentatie

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For Research Use Only. Not for use in diagnostic procedures.