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  5. OPTIZYME™ M-MLV Reverse Transcriptase, Fisher BioReagents™

OPTIZYME™ M-MLV Reverse Transcriptase, Fisher BioReagents™

72.45 - 148.40

Specificatie

Concentratie 200 U/μL
Kleur Colorless
pH 8.3
Voor gebruik met (toepassing) Generation of labeled cDNA probes for microarrays, synthesis of cDNA for cloning and expression, analysis of RNA by primer extension, DNA labeling, optimum activity at 42°C, incorporates modified Nucleotides
Opslagbuffer OPTIZYME™ M-MLV RT Buffer 5X : Tris HCl 250mM (8.3 pH at 25°C), KCl 250mM, MgCl2 20mM, DTT 50mM
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Produktcode Merk Aantal Prijs Hoeveelheid en beschikbaarheid  
Produktcode Merk Aantal Prijs Hoeveelheid en beschikbaarheid  
12827162
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Fisher BioReagents
BP8104-1 		5000 U N/A N/A N/A
12837162
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Fisher BioReagents
BP8104-5 		25,000 U N/A N/A N/A
Beschrijving

Beschrijving

OPTIZYME™ M-MuLV RT is a genetically modified MLV RT that possesses RNA- and DNA-dependent polymerase activity.

Applications and Features:

  • OPTIZYME M-MLV RT is used for efficient first strand cDNA synthesis for RT-PCR and real-time RT-PCR (up to 13kb)
  • Generation of labeled cDNA probes for microarrays
  • Synthesis of cDNA for cloning and expression
  • Analysis of RNA by primer extension
  • DNA labeling
  • Optimum activity at 42°C (active up to 50°C)
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)

Supplied with:
5X OPTIZYME M-MLV RT Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 250mM KCl, 20mM MgCl2, 50mM DTT
Source: E. Coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase
Quality Control:

  • Endo-, exodeoxyribonuclease, phosphatase and ribonuclease-free
  • Functionally tested in first strand cDNA and RT-PCR

Specificatie

Specificatie

200 U/μL
8.3
OPTIZYME™ M-MLV RT Buffer 5X : Tris HCl 250mM (8.3 pH at 25°C), KCl 250mM, MgCl2 20mM, DTT 50mM
E. coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus Reverse Transcriptase
Liquid
Colorless
Generation of labeled cDNA probes for microarrays, synthesis of cDNA for cloning and expression, analysis of RNA by primer extension, DNA labeling, optimum activity at 42°C, incorporates modified Nucleotides
-20°C
OPTIZYME™ M-MLV Reverse Transcriptase
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Veiligheid en behandeling

Veiligheid en behandeling

Productidentificatie
  • OPTIZYME

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Documentatie

Documentatie

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