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Macherey-Nagel™ NucleoSpin™ TriPrep, Mini kit for RNA, DNA, and Protein Purification
Parallel isolation of RNA, DNA and, protein from an undivided sample.
Merk: Macherey-Nagel™ 740966.50
This item is not returnable.
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Beschrijving
NucleoSpin™ miRNA is suitable for the parallel isolation of small RNA, large RNA and protein.
Superior selectivity - purify your RNA fractionated by size
Isolation of small RNA only (18 to 200 bases)Isolation of small (18 to 200 bases) and large (>200 bases) RNA in two fractionsIsolation of total RNA (small and large in one fraction)
Additional isolation of total protein
Denatured protein ready-to-use for analysis in SDS-PAGE and Western blot.
Excellent RNA recovery - even without phenol/chloroform
Lysis with chaotropic saltSpin column based procedure
Convenient handling - complete equipment provided
NucleoSpin filters - efficient sample homogenisationNucleoSpin protein removal columns - highest purity of small RNArDNase - highly efficient on-column digestion of genomic DNA at RT
- Silica membrane technology
- Format: minispin columns
- Sample size: <107 cultured cells, <30mg human/tissue; <50 mg plant tissue, <150μL reaction mixture
- Fragment size, small RNA: 18 to 200 bases
- Fragment size, large RNA: >200 bases
- Typical yield: 10μg small RNA; 95μg large RNA from 107 HeLa cells
- Binding capacity: 200μg
- Elution volume, 30μL to 100μL
- Preparation time: <45min (6 preps human/animal tissue, small and large RNA); <35min (6 preps human/animal tissue, small RNA only)
- Small RNA (18 to 200 bases)
- Large RNA (>200 bases)
- Total RNA (small and large RNA in one fraction), and isolate your total protein fraction For small and large RNA and total RNA purification, and isolation of total protein fraction.
Specificatie
| Shredders | |
| 1 Ea. | |
| Spin Column |
| NucleoSpin TriPrep | |
| With 2mL/1.5mL Collection Tubes, NucleoSpin Filters, buffers, RNase-free rDNase | |
| Rapid purification of total RNA, DNA, and protein from small and precious samples |
Veiligheid en behandeling
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- Gevaar
Gevarencategorie
- Ontvlambare vloeistof Categorie 3
- Acute toxiciteit Categorie 4
- Huidcorrosie/-irritatie Indeling Categorie 2
- Ernstig oogletsel/oogirritatie Categorie 2
- Sensibilisatie van de luchtwegen Categorie 1
- AQUATISCH GEVAAR OP LANGE TERMIJN Categorie 3 voor chronische toxiciteit
Gevarenaanduiding
- H226-Ontvlambare vloeistof en damp.
- H302-Schadelijk bij inslikken.
- H315-Veroorzaakt huidirritatie.
- H319-Veroorzaakt ernstige oogirritatie.
- H334-Kan bij inademing allergie- of astmasymptomen of ademhalingsmoeilijkheden veroorzaken.
- H412-Schadelijk voor in het water levende organismen, met langdurige gevolgen.
Veiligheidsmaatregel
- P261-Inademing van stof/rook/gas/nevel/damp/spuitnevel vermijden.
- P342+P311-Bij ademhalingssymptomen: Een ANTIGIFCENTRUM/arts/ raadplegen.
Aanvullende informatie
- MIXTURE LIST-Bevat: zinc chloride; guanidinium thiocyanate,ethanol (denatured with 1%IPA/1%MEK, acc.2016/1867/EU); rDNa
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