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Macherey-Nagel™ NucleoSpin™ DNA Stool, Mini kit for DNA from stool
Combines enzymatic lysis with mechanical disruption of cell walls for efficient isolation of high-quality genomic DNA from any stool sample, fresh or frozen. Complete kit includes NucleoSpin™ Bead Tubes.
Merk: Macherey-Nagel™ 740472.250
1386.60 EUR valid until 2025-12-31
Use promo code "25339" to get your promotional price.
Beschrijving
Isolation of genomic DNA from bacterial and epithelial cells from stool samples
- Efficient procedure combining enzymatic lysis and bead disruption
- Isolation of high-quality genomic DNA (microbial and host) from fresh and frozen samples
- Suitable for any stool sample – carnivores, omnivores, and herbivore
- Bead Tubes included for efficient lysis – compatible with most common disruption devices
- NucleoSpin™ Inhibitor Removal Columns ensure highest purity of the isolated DNA
- Downstream applications: PCR, real-time PCR, Southern blotting, microarray technology
Specificatie
Room temperature (18 - 25 °C) | |
Silica MembraneTechnology and Mechanical (Bead Tube) Disruption | |
Binding capacity: 50 μg | |
Disruption device (e.g. Vortex-Genie 2 with MN Bead Tube Holder), Centrifuge, Vortex mixer | |
DNA Stool Columns, Bead Tubes Type A, Inhibitor Removal Columns, Collection Tubes (2 mL), Collection Tubes (2 mL, lid), Buffers | |
A260/A280: 1.7 - 1.9 | |
Fragment size: 200 bp - approx. 50 kbp | |
Varies by sample type, quality and water content |
Mini Spin Column | |
Stool Samples (Herbivores, Omnivores, or Carnivores, Fresh or Frozen) | |
Elution volume: 30 - 100 μL | |
Isolation of genomic DNA from stool samples | |
250 | |
Approx. 200 mg (180 - 220 mg) | |
60 min/10 prep |
Veiligheid en behandeling
- NucleoSpin DNA Stool
Signaalwoord
- Waarschuwing
Gevarencategorie
- Acute toxiciteit Categorie 4
- AQUATISCH GEVAAR OP LANGE TERMIJN Categorie 3 voor chronische toxiciteit
- Ernstig oogletsel/oogirritatie Categorie 2
- Ontvlambare vloeistof Categorie 3
- Huidcorrosie/-irritatie Indeling Categorie 2
- Dzia anie toksyczne na narzady docelowe Categorie 3
Gevarenaanduiding
- H226-Ontvlambare vloeistof en damp.
- H302-Schadelijk bij inslikken.
- H315-Veroorzaakt huidirritatie.
- H319-Veroorzaakt ernstige oogirritatie.
- H336-Kan slaperigheid of duizeligheid veroorzaken.
- H412-Schadelijk voor in het water levende organismen, met langdurige gevolgen.
Aanvullende informatie
- MIXTURE LIST-Bevat: 2-propanol, guanidine hydrochloride
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