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Macherey-Nagel™ NucleoSpin™ 96 PCR Clean-up, 96-well kit for PCR clean up
NucleoSpin™ 96 Gel and PCR Clean-Up Kit provides time saving medium to high throughput PCR clean up.
Merk: Macherey-Nagel™ 740658.2
This item is not returnable.
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Beschrijving
The NucleoSpin™ 96 PCR Clean-up kit allows direct clean-up of PCR reaction mixtures. Within the procedure the addition of chaotropic salt (Buffer NT) allows a reversible adsorption of the PCR products to the silica membrane of the NucleoSpin™ 96 PCR Clean-up Binding Plates. High purity of the PCR-products is achieved by complete removal of primers, primer-dimers, salts, nucleotides, and proteins (e.g., polymerases, BSA) in subsequent washing steps using Buffer NT3. Highly pure PCR products are finally eluted with Elution Buffer NE (5 mM Tris/HCl, pH 8.5) or water (pH 8.5), and can be used directly for further applications.
- Silica membrane technology
- Processing: manual/automated, vacuum/centrifugation
- Sample material: < 10 0μL PCR reaction mixture
- Elution volume: 75 to 150 μL
- Binding capacity: 15 μg
- Preparation time: 45 min./plate
- Fragment size: 65 bp to 10 kb
Specificatie
| 18°C to 25°C | |
| PCR clean up | |
| <100 μL |
| 96-well Plate | |
| 192 Preps |
Veiligheid en behandeling
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Aanvullende informatie
- Waarschuwing
Gevarencategorie
- Acute toxiciteit Categorie 4
- AQUATISCH GEVAAR OP LANGE TERMIJN Categorie 3 voor chronische toxiciteit
Gevarenaanduiding
- H302-Schadelijk bij inslikken.
- H412-Schadelijk voor in het water levende organismen, met langdurige gevolgen.
Aanvullende informatie
- MIXTURE LIST-Bevat: guanidinium thiocyanate
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