Macherey-Nagel™ NucleoMag Tissue kit for DNA purification from cells and tissue

Beschrijving

NucleoMag 96 Tissue is designed for the rapid parallel purification of genomic DNA from animal tissues, cell cultures and bacteria in a 96 well format. NucleoMag 96 Trace is designed for the purification of DNA from forensic samples (e.g., buccal swabs, dried blood spots, cigarette filters). NucleoMag 96 Plant is designed for the purification of genomic DNA from plant tissues in a 96 well format. The magnetic bead technology provides a closed system therefore, the risk for cross contamination is greatly reduced. The NucleoMag technology can be easily adapted to common laboratory automation platforms. It can be used either with static-pin separators (eg NucleoMag SEP) or magnetic separators integrated into robotic workstations. Kit components: NucleoMag 96 Tissue – NucleoMag B-beads, buffers, Proteinase K; NucleoMag 96 Trace – NucleoMag B-beads, buffers, Proteinase K; NucleoMag 96 Plant – NucleoMag C-beads, buffers.

• Rapid, economic purification of genomic DNA using highly reactive superparamagnetic beads, easily adapted to automated use
• Sample size: NucleoMag 96 Tissue – up to 20mg animal tissue, 1 x 10⁷ cells or bacteria NucleoMag 96 Trace – buccal swabs, dried blood spots, cigarette filters NucleoMag 96 Plant – 20mg to 50 mg plant tissue
• Typical yield: NucleoMag 96 Tissue/Plant – 10μg to 20μg DNA NucleoMag 96 Trace – 1μg to 3μg DNA
• A_260/280 ≥1.6 to 1.9
• Concentration of sample possible
• Complete removal of PCR* inhibitors
• Small elution volumes (≥50μL)
• Yield does not depend on elution volume
• DNA ready to use in downstream applications
• Binding capacity: 0.4μg DNA per μL of NucleoMag bead suspension
• Size of beads: 1μm to 3μm
• Reduced carry-over of beads For the manual and automated purification of gDNA from a wide range of souces.