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Macherey-Nagel™ NucleoBond™ PC EF, kit for endotoxin-free plasmid DNA
Removes endotoxins via an additional washing step, without ice incubation. Macherey-Nagel™ NucleoBond™ PC EF Kit is a classic anion exchanger for purification of endotoxin-free plasmid DNA. The kit includes NucleoBond™ AX Columns, all buffers, RNase A and NucleoBond™ Filters.
Merk: Macherey-Nagel™ 740548
This item is not returnable.
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Beschrijving
An important prerequisite for transfections and gene therapy applications is endotoxin-free plasmid DNA. The patented procedure of NucleoBond™ PC EF reduces endotoxins to a very low level using the same working procedure as the standard protocol. In comparison to competitive kits, there is no need for a time-consuming incubation step to remove the endotoxins.
Features
- Fast and easy clarification by NucleoBond™ Folded Filters—No centrifugation required, no shearing forces
- Proven anion-exchange technology—Yields transfection-grade high-copy plasmid DNA from E. coli
- NucleoBond™ Columns contain NucleoBond™ AX Silica Resin packed between inert filters
- Polypropylene columns are resistant to organic solvents and are free of DNase and RNase
- AX Resin can be used over a wide pH range and can remain in contact with buffers for up to three hours
Specifications
- DNA with less than 0.1 EU/μg DNA
- Purity of plasmid DNA: A260/A280 = 1.80–1.95
- Recovery of plasmid DNA >90%
- Structural integrity >90%
- Yield between 500μg up to 10mg, depending on kit used
Specificatie
| Endotoxin level: <0.1 EU/μg | |
| Anionuitwisselingschromatografie | |
| High-copy plasmids from E. coli | |
| 5 | |
| 500 - 2 000 ml | |
| Yes | |
| <300 kbp | |
| 2,000–10,000μg |
| Gravity-flow Column (Giga) | |
| Recovery of plasmid DNA: >90% | |
| NucleoBond AX 10000 Columns, buffers, RNase A, NucleoBond Bottle Top Filters | |
| NucleoBond™ PC 10,000 EF | |
| A260/A280: 1.80–1.95 | |
| 500 - 2 000 ml | |
| 180 min |
Veiligheid en behandeling
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- Gevaar
Gevarencategorie
- Ontvlambare vloeistof Categorie 3
- Huidcorrosie/-irritatie Indeling Categorie 2
- Ernstig oogletsel/oogirritatie Categorie 2
- Sensibilisatie van de luchtwegen Categorie 1
Gevarenaanduiding
- H226-Ontvlambare vloeistof en damp.
- H315-Veroorzaakt huidirritatie.
- H319-Veroorzaakt ernstige oogirritatie.
- H334-Kan bij inademing allergie- of astmasymptomen of ademhalingsmoeilijkheden veroorzaken.
Veiligheidsmaatregel
- P210-Verwijderd houden van warmte, hete oppervlakken, vonken, open vuur en andere ontstekingsbronnen. Niet roken.
- P261-Inademing van stof/rook/gas/nevel/damp/spuitnevel vermijden.
- P342+P311-Bij ademhalingssymptomen: Een ANTIGIFCENTRUM/arts/ raadplegen.
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
Aanvullende informatie
- MIXTURE LIST-Bevat: ethanol (diluted < 20 %)/sodium hydroxide solution (diluted< 2 %)/dodecyl sulfate, sodium salt
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