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  5. Macherey-Nagel™ NucleoBond™ PC EF, kit for endotoxin-free plasmid DNA
  6. 12863931

Macherey-Nagel™ NucleoBond™ PC EF, kit for endotoxin-free plasmid DNA

Removes endotoxins via an additional washing step, without ice incubation. Macherey-Nagel™ NucleoBond™ PC EF Kit is a classic anion exchanger for purification of endotoxin-free plasmid DNA. The kit includes NucleoBond™ AX Columns, all buffers, RNase A and NucleoBond™ Filters.

Merk:  Macherey-Nagel™ 740550


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Produktcode. 12863931

  • 304.00 /

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Beschrijving

Beschrijving

An important prerequisite for transfections and gene therapy applications is endotoxin-free plasmid DNA. The patented procedure of NucleoBond™ PC EF reduces endotoxins to a very low level using the same working procedure as the standard protocol. In comparison to competitive kits, there is no need for a time-consuming incubation step to remove the endotoxins.

Features

  • Fast and easy clarification by NucleoBond™ Folded Filters—No centrifugation required, no shearing forces
  • Proven anion-exchange technology—Yields transfection-grade high-copy plasmid DNA from E. coli
  • NucleoBond™ Columns contain NucleoBond™ AX Silica Resin packed between inert filters
  • Polypropylene columns are resistant to organic solvents and are free of DNase and RNase
  • AX Resin can be used over a wide pH range and can remain in contact with buffers for up to three hours

Specifications

  • DNA with less than 0.1 EU/μg DNA
  • Purity of plasmid DNA: A260/A280 = 1.80–1.95
  • Recovery of plasmid DNA >90%
  • Structural integrity >90%
  • Yield between 500μg up to 10mg, depending on kit used
Specificatie

Specificatie

Endotoxin level: <0.1 EU/μg
Anion Exchange Chromatography Technology
High-copy plasmids from E. coli
10
1 Kit
Yes
<300 kbp
100–500μg
Maxi Gravity-flow Column
Recovery of plasmid DNA: >90%
NucleoBond AX 500 Columns, buffers, RNase A, NucleoBond Folded Filters XL
NucleoBond™ PC 500 EF
A260/A280: 1.80–1.95
30 - 150mL
100 min./2 preps
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