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Macherey-Nagel™ NucleoBond™ PC 10000, Giga kit for transfection-grade plasmid DNA

1st generation anion exchanger for purification of plasmid DNA.

Merk:  Macherey-Nagel™ 740593


Produktcode. 12877521

  • 481.00 /

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Beschrijving

Beschrijving

NucleoBond™ kits utilize anion-exchange chromatography with optimized lysis buffer volumes for plasmid DNA purification. PC 10,000 kits are recommended for the isolation of high-copy plasmids (>20 copies/cell). Low-copy plasmids (<20 copies/cell) can be isolated as well, but this requires additional buffers.

  • Folded Filters are included for fast and easy clarification of lysates with no centrifugation required and no shearing forces
  • Contains sufficient buffer to perform 5 giga preps, with typical yields of 2,000–10,000μg of plasmid DNA from E. coli culture
  • Columns are resistant to organic solvents and are free of DNase and RNase
  • AX Resin can be used over a wide pH range (2.5–8.5) and can remain in contact with buffers for up to three hours
Specificatie

Specificatie

Yes
Anion Exchange Chromatography Technology
Purification of high-copy and low-copy plasmids
5 Preps
1 Kit
500—2000mL (HC)
120–150 min/2 preps
Gravity-flow Column (Giga)
E. coli Culture
Resuspension Buffer S1 (750mL), Lysis Buffer S2 (750mL), Neutralization Buffer S3 (750mL), Equilibration Buffer N2 (500/125mL), Wash Buffer N3 (1000/125mL), Elution Buffer N5 (500/125mL), RNase A lyophilized (75mg), NucleoBond AX 10000 Columns (5), NucleoBond Folded Filters Type 1 (10), NucleoBond Folded Filters Type 2 (10)
PC 10000 Columns
A260/A280: 1.80–1.95
<300 kbp
2,000–10,000μg
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