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Invitrogen™ PureLink™ Quick Plasmid Miniprep Kit
The PureLink Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in about 30 minutes, using silica membrane columns.
117.00 - 486.00
Specificatie
| Concentratie | 20 mg/mL |
|---|---|
| Kolomtype | Spin Column |
| Content And Storage | • Resuspension buffer • RNase A (20 mg/mL) • Lysis buffer • Precipitation buffer • Wash buffers • TE buffer • Spin columns in wash tubes • Elution tubes |
| Indeling | Spin Column |
| Isolation Technology | Silica Spin Column |
Inclusief: Resuspension Buffer (R3)
RNase A (20 mg/mL)
Lysis Buffer (L7)
Precipitation Buffer (N4)
Wash Buffer (W9)
Wash Buffer (W10)
TE Buffer (TE)
Spin Columns
Wash and Recovery Tubes
| Produktcode | Merk | Aantal reacties | Prijs | Hoeveelheid en beschikbaarheid | |||||
|---|---|---|---|---|---|---|---|---|---|
| Produktcode | Merk | Aantal reacties | Prijs | Hoeveelheid en beschikbaarheid | |||||
|
10553293
|
Invitrogen™
K210010 |
50 Preps |
117.00
|
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|
10694823
|
Invitrogen™
K210011 |
250 Preps |
486.00
|
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Beschrijving
The PureLink™ Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in 30-45 minutes, using silica membrane columns.
Features of the PureLink™ Quick Plasmid Miniprep Kit:
- Fast—ready-to-use plasmid DNA typically in 30-45 minutes
- Flexible—formats work with vacuum and centrifugation platforms
Reliable and cost-effective isolation of plasmid DNA
The PureLink™ Quick Plasmid Miniprep Kit allows isolation of high-quality plasmid DNA from 1–5 mL cultures in 30-45 minutes, using the PureLink™ spin columns. These columns use a unique silica membrane to achieve high yields of up to 40 μg of sequencing-grade plasmid DNA. Cells are lysed using an alkaline/SDS procedure and the lysate is applied to a silica membrane column that selectively binds plasmid DNA. Contaminants are removed with Wash Buffer, and the plasmid DNA is eluted in TE Buffer. The eluted DNA is suitable for all routine downstream applications.
Compatible with flexible formats
The PureLink™ Quick Plasmid Miniprep Kit can be used with a centrifuge or a vacuum manifold for purifying plasmid DNA. Typically, DNA isolated using the PureLink™ Quick Plasmid Miniprep Kit has an A260/A280 of >1.80 when samples are diluted in Tris-HCl (pH 7.5), indicating that the DNA is reasonably free of proteins that could interfere with downstream applications.
Isolated DNA can be used for a variety of downstream applications
Plasmid DNA purified using PureLink™ Quick Plasmid Miniprep Kit is suitable for all routine downstream applications, including bacterial and mammalian cell transformations, DNA sequencing, restriction enzyme digestion, cloning, and PCR.
Informatie over bestelling
Shipping Condition: Room Temperature
Specificatie
| 20 mg/mL | |
| • Resuspension buffer • RNase A (20 mg/mL) • Lysis buffer • Precipitation buffer • Wash buffers • TE buffer • Spin columns in wash tubes • Elution tubes |
|
| Silica Spin Column | |
| Plasmid DNA | |
| Not High-throughput Compatible (Manual) | |
| PureLink™ | |
| Room Temperature | |
| 30 to 45 min. |
| Spin Column | |
| Spin Column | |
| Bacterial Culture | |
| Next-Generation Sequencing, Cloning, Sequencing, Nucleic Acid Labeling, PCR, Transformation | |
| < 100 μg (Small-Scale) Plasmid DNA | |
| Quick Plasmid MiniPrep Kit | |
| Plasmid DNA |
Veiligheid en behandeling
Productidentificatie
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- PureLink Quick Plasmid Miniprep Kit
Signaalwoord
- Waarschuwing
Gevarencategorie
- Acute toxiciteit Categorie 4
- Huidcorrosie/-irritatie Indeling Categorie 2
- Ernstig oogletsel/oogirritatie Categorie 2
Gevarenaanduiding
- H226-Ontvlambare vloeistof en damp.
Veiligheidsmaatregel
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
- P264-Na het werken met dit product grondig wassen.
- P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
- P337+P313-Bij aanhoudende oogirritatie: een arts raadplegen.
- P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
- P332+P313-Bij huidirritatie: een arts raadplegen.
- P301+P312-NA INSLIKKEN: bij onwel voelen een ANTIGIFCENTRUM / arts/ raadplegen.
Aanvullende informatie
- MIXTURE LIST-Bevat: Sodium azide, Sodiumhydroxide, Guanidine hydrochloride, Guanidine hydrochloride
For Research Use Only. Not for use in diagnostic procedures.
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