missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Molecular Biology Reagents and Kits
  4. DNA Extraction and Purification
  5. Invitrogen™ PureLink™ HiPure Plasmid Miniprep Kit

Invitrogen™ PureLink™ HiPure Plasmid Miniprep Kit

The PureLink HiPure Plasmid Miniprep Kit allows isolation of high yields of highly pure plasmid DNA from E.coli in less than 2 hours, without use of any organic solvents or cesium chloride, and contains Low endotoxin levels.

173.00 - 566.00

Specificatie

Indeling Kit
Schaalverdeling Mini
Monstertype Bacterial Culture
Type eindproduct BAC DNA, Plasmid DNA
Voor gebruik met (toepassing) Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Meer specificaties weergeven

Producten 2
Produktcode Merk Aantal reacties Includes Prijs Hoeveelheid en beschikbaarheid  
Produktcode Merk Aantal reacties Includes Prijs Hoeveelheid en beschikbaarheid  
10013552
Bekijk documenten
missing translation for 'viewProductCertificates'
Invitrogen™
K210002 		25 Preps
173.00
Please to purchase this item. Need a web account? Register with us today!
10770394
Bekijk documenten
missing translation for 'viewProductCertificates'
Invitrogen™
K210003 		100 Preps
566.00
Please to purchase this item. Need a web account? Register with us today!
Beschrijving

Beschrijving

The PureLink HiPure Plasmid Miniprep Kit allows isolation of high yields of highly pure plasmid DNA from E.coli in less than 2 hours, without use of any organic solvents or cesium chloride, and contains Low endotoxin levels.

Plasmid DNA of High Purity

  • PureLink HiPure Plasmid Miniprep Kit is designed to efficiently isolate plasmid DNA from E.coli in 1.5–2 hours
  • Kit employs anion-exchange resin composed of small particles with a uniform pore size to purify plasmid DNA to a level equivalent to two passes through CsCl gradients
  • Need for additional steps to remove contaminants such as phenol, chloroform, and CsCl are eliminated, minimizing exposure to and disposal of hazardous materials
  • Efficient—Increased yields with low endotoxin Levels

High Yield and Efficient Endotoxin Removal

  • PureLink HiPure Plasmid Miniprep Kit allows purification of up to 30 μg of high quality plasmid DNA from 1–3 mL overnight E.coli cultures in approximately 1 hour, with an endotoxin level of 0.1–1 EU/μg, when cloning high copy number plasmids
  • Typically, DNA isolated using the PureLink HiPure Plasmid Miniprep Kit has an A260/A280 >1.80 when samples are diluted in Tris-HCl (pH 7.5) indicating that the DNA is reasonably free of proteins that could interfere with downstream applications

Isolated DNA can be used for a Number of Downstream Applications:

  • Purified DNA is suitable for downstream applications, including those requiring the highest purity DNA, such as mammalian transfections, automated and manual DNA sequencing, PCR amplification, in vitro transcription, bacterial cell transformation, cloning, and labeling

Informatie over bestelling

Shipping Condition: Room Temperature

Specificatie

Specificatie

Kit
Bacterial Culture
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
< 100 μg (Small-Scale) Plasmid DNA
Plasmid MiniPrep Kit
BAC DNA, Plasmid DNA
Mini
BAC DNA, Plasmid DNA
Not High-throughput Compatible (Manual)
PureLink™
Room Temperature
2 hr.
Video's
Veiligheid en behandeling

Veiligheid en behandeling

Productidentificatie
  • PureLink HiPure Plasmid Miniprep Kit

Signaalwoord
  • Waarschuwing

Gevarencategorie
  • Ernstig oogletsel/oogirritatie Categorie 2
  • Huidcorrosie/-irritatie Indeling Categorie 2

Gevarenaanduiding
  • H315-Veroorzaakt huidirritatie.
  • H319-Veroorzaakt ernstige oogirritatie.

Veiligheidsmaatregel
  • P264-Na het werken met dit product grondig wassen.
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.
  • P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
  • P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
  • P332+P313-Bij huidirritatie: een arts raadplegen.
  • P337+P313-Bij aanhoudende oogirritatie: een arts raadplegen.
  • P362+P364-Verontreinigde kleding uittrekken en wassen alvorens deze opnieuw te gebruiken.

Aanvullende informatie
  • MIXTURE LIST-Bevat: Sodium lauryl sulfate, sodium hydroxide

SDS
Documentatie

Documentatie

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.