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Invitrogen™ PureLink™ HiPure Plasmid Maxiprep Kit
The PureLink HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli.
191.00 - 556.00
Specificatie
| Indeling | Column |
|---|---|
| Isolation Technology | Anion Exchange Resin |
| Schaalverdeling | Maxi |
| Monstertype | Bacterial Culture |
| Elutievolume | 15 mL |
| Produktcode | Merk | Aantal | Includes | Prijs | Hoeveelheid en beschikbaarheid | ||||
|---|---|---|---|---|---|---|---|---|---|
| Produktcode | Merk | Aantal | Includes | Prijs | Hoeveelheid en beschikbaarheid | ||||
|
10257072
|
Invitrogen™
K210006 |
10 Preps |
239.00
|
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|
12809702
|
Invitrogen™
K210006 |
10 Preps | N/A | ||||||
|
10464402
|
Invitrogen™
K210007 |
25 Preps |
556.00
|
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Beschrijving
The PureLink HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Maxiprep Kit protocol will typically yield 500–850 μg plasmid DNA from 100–200 mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients.
- Labor and time saving- no need for extra steps to remove endotoxin and other contaminants
- High-purity, low-endotoxin product- pure enough for mammalian cell transfection
- Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs
Anion-exchange chromatography for plasmid purification
- PureLink HiPure Plasmid DNA Purification Kits use patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients
- Resin combines excellent capacity with a fast flow rate, high resolution, high yield, and efficient endotoxin removal
- Plasmid preparations can typically be completed in less than 2 hours
Contaminant-free DNA
- Unlike cesium chloride gradient protocols, PureLink HiPure Plasmid Purification Systems do not use troublesome solvents to work with and to dispose of
- Typically, plasmid DNA prepared using the PureLink HiPure Plasmid Midiprep Kit will have an A260/A280 ratio of >1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications
- Endotoxin levels are typically within 0.1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection
- Purification time is two hours
Informatie over bestelling
Shipping Condition: Room Temperature
Specificatie
| Column | |
| Maxi | |
| 15 mL | |
| Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription | |
| <40kb, Low Copy Plasmid, High Copy Plasmid, BAC | |
| PureLink™ | |
| Transfection Grade | |
| BAC DNA, Plasmid DNA | |
| 2 hr. |
| Anion Exchange Resin | |
| Bacterial Culture | |
| BAC DNA, Plasmid DNA | |
| Not High-throughput Compatible (Manual) | |
| > 200 μg (Large-Scale) Plasmid DNA | |
| Plasmid MaxiPrep Kit | |
| Room Temperature | |
| PureLink™ | |
| 1000 μg |
Veiligheid en behandeling
Productidentificatie
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- PureLink HiPure Plasmid Maxiprep Kit
Signaalwoord
- Waarschuwing
Gevarencategorie
- Ernstig oogletsel/oogirritatie Categorie 2
- Huidcorrosie/-irritatie Indeling Categorie 2
Gevarenaanduiding
- H315-Veroorzaakt huidirritatie.
- H319-Veroorzaakt ernstige oogirritatie.
Veiligheidsmaatregel
- P264-Na het werken met dit product grondig wassen.
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
- P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
- P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
- P332+P313-Bij huidirritatie: een arts raadplegen.
- P337+P313-Bij aanhoudende oogirritatie: een arts raadplegen.
- P362+P364-Verontreinigde kleding uittrekken en wassen alvorens deze opnieuw te gebruiken.
Aanvullende informatie
- MIXTURE LIST-Bevat: acetic acid
For Research Use Only. Not for use in diagnostic procedures.
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