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  6. Cytiva HisTrap™ FF Columns: 16 x 25mm
  7. 11595674

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Merk:  Cytiva 17-5319-02


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Produktcode. 11595674

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Beschrijving

Beschrijving

  • Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
  • Column material: polypropylene
  • Bed dimensions: 16 × 25mm
  • Flow velocity: <20mL/min.
  • Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
  • Compatible with wide range of reducing agents, detergents, denaturants, and other additives
  • Simple operation with syringe, perstaltic pump, or chromatographic system
  • Columns may be connected in series to increase capacity

Medium
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

5 mL
16 mm
<20 mL/min.
3 to 12
4°C to 30°C, 20% Ethanol
100 x 1 mL
0.6 in.
HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Polypropylene
6% Cross-linked Agarose
5 bar (0.5 MPa, 70 psi)
Pre-packed Column
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Gevarencategorie
  • Ontvlambare vloeistof Categorie 3

Gevarenaanduiding
  • H226-Ontvlambare vloeistof en damp.
  • EUH208-Bevat . Kan een allergische reactie veroorzaken.

Veiligheidsmaatregel
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.
  • P210-Verwijderd houden van warmte, hete oppervlakken, vonken, open vuur en andere ontstekingsbronnen. Niet roken.
  • P241-Explosieveilige elektrische/ventilatie-/verlichtings-/ apparatuur gebruiken.
  • P303+P361+P353-BIJ CONTACT MET DE HUID (of het haar): verontreinigde kleding onmiddellijk uittrekken. Huid met water afspoelen/afdouchen.
  • P235-Koel bewaren.
  • P501-Inhoud/verpakking afvoeren naar .

Aanvullende informatie
  • MIXTURE LIST-Bevat: ethanol, Nickel

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