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Invitrogen™ GeneArt™ Genomic Cleavage Detection Kit

The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation.

Merk:  Invitrogen™ A24372

228.80 EUR valid until 2025-06-30
Use promo code "24108" to get your promotional price.


Produktcode. 15396351

  • 260.00 /

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Waarschuwingen:

To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price


Inclusief:
  • 1 bottle Cell Lysis Buffer
  • 1 tube Proteinase K
  • 1 tube PCR Supermix
  • 1 tube Water
  • 1 tube Detection Enzyme
  • 1 tube Detection Reaction Buffer
  • 1 tube Control Template & Primers
This item is not returnable. View return policy

Beschrijving

Beschrijving

The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation. When using genome editing tools to obtain targeted mutations, it is necessary to determine the efficiency at which these nucleases cleave the target sequence, particularly prior to proceeding to the more laborious and expensive processes of cloning and sequencing.

The GeneArt genomic cleavage detection kit contains all of the reagents required prior to gel analysis. A sample of the edited cell population is used as a direct PCR template for amplification with primers specific to the targeted region. The PCR product is then denatured and re-annealed to produce heteroduplex mismatches where double strand breaks have occurred, resulting in indel introduction. These mismatches are recognized and cleaved by the detection enzyme. This cleavage is both easily detectable and quantifiable using gel analysis.

  • Easy—no genomic DNA isolation, direct PCR amplification

  • Rapid—4 hour total processing time

  • Quantative—gel band density directly correlated to target indel introduction

Recommended Storage

Store all at -5 to -30°C

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

Contains:

• 1 bottle Cell Lysis Buffer
• 1 tube Proteinase K
• 1 tube PCR Supermix
• 1 tube Water
• 1 tube T7E1 Detection Enzyme
• 1 tube T7E1 Detection Reaction Buffer
• 1 tube Control Template & Primers

Store all components at -5° to -30°C.
Liquid
Taq Polymerase
CRISPR-Cas9, TAL Effector Nuclease
20 reactions
20 Reactions
Primer-probe
Kit
Fast
Genomic Cleavage Detection Kit
INDEL
20 Reactions
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Productidentificatie
  • GeneArt Genomic Cleavage Detection Kit

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Gevarencategorie
  • Ernstig oogletsel/oogirritatie Categorie 2
  • Sensibilisatie van de luchtwegen Categorie 1

Gevarenaanduiding
  • H319-Veroorzaakt ernstige oogirritatie.
  • H334-Kan bij inademing allergie- of astmasymptomen of ademhalingsmoeilijkheden veroorzaken.

Veiligheidsmaatregel
  • P261-Inademing van stof/rook/gas/nevel/damp/spuitnevel vermijden.
  • P264-Na het werken met dit product grondig wassen.
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.
  • P284-¢Bij ontoereikende ventilatie! adembescherming dragen.
  • P304+P340-NA INADEMING: de persoon in de frisse lucht brengen en ervoor zorgen dat deze gemakkelijk kan ademen.
  • P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
  • P337+P313-Bij aanhoudende oogirritatie: een arts raadplegen.
  • P342+P311-Bij ademhalingssymptomen: Een ANTIGIFCENTRUM/arts/ raadplegen.
  • P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.

Aanvullende informatie
  • MIXTURE LIST-Bevat: Poly(oxy-1,2-ethanediyl) .alpha.-[4-(1,1,3,3-tetramethylbutyl)phenyl]-.omega.-hydroxy, Proteinase

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