Flag tag Mouse anti-Tag, Clone: 2B3C4, Proteintech

Beschrijving

Flag Tag Antibody is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more Flag tags, independently on N-terminal, C-terminal or internal regions of the target protein.

DDK (FLAG-tag, FLAG octapeptide, FLAG epitope) is a polypeptide protein tag that can be added to a protein using recombit DNA technology, having the sequence motif DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. It has been used to separate recombit, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.