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Cytiva Ficoll PM400
Sucrosepolymeren met hoog moleculair gewicht, gevormd door copolymerisatie van sucrose met epichloorhydrine. GE Healthcare™ Ficoll PM400 is een synthetische, neutrale, sterk vertakte, hydrofiele polymeer van sucrose, gebruikt voor de productie van dichtheidsgradiënten voor de scheiding van cellen en subcellulaire bestanddelen door centrifugatie of door sedimentatie bij de zwaartekrachteenheid.
Merk: Cytiva 17-0300-10
This item is not returnable.
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Beschrijving
- For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity
- Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution
- Concentrations of up to 50% (w/v) covering densities of up to 1.2g/mL can be achieved
- Has better osmotic properties than sucrose
- Preserves functional and morphological integrity
- Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size
- Does not penetrate biological membranes
- Serves as the raw material for preparation of Ficoll-Paque gradients
- Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier
- Supplied as dry powders
- Used in the formation of density gradients for the separation and isolation of eukaryotic cells, organelles, and bacterial cells, as a stabilizing agent and as a separation medium for the isolation of lymphocytes
- Used in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies
- Used for gradient centrifugation in all types of centrifuge rotors and for separation at unit gravity

Specificatie
100 g | |
Zorgt voor dichtheidsgraden om cellen en subcellulaire componenten te scheiden | |
0.12 EU/mg | |
Centrifugation Media | |
Powder |
4 °C tot 30 °C | |
≈17 ml/g | |
White | |
1.2 g/ml |
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