missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Restriction Enzymes
  5. Thermo Scientific™ Mph1103I (NsiI) (10 U/μL)
  6. 10304720

Thermo Scientific™ Mph1103I (NsiI) (10 U/μL)

The Mph1103I (NsiI) restriction enzyme recognizes ATGCA^T sites and cuts best at 37°C in R buffer (Isoschizomers: EcoT22I, NsiI, Zsp2I).

Merk:  Thermo Scientific™ ER0731

64.60 EUR valid until 2025-06-30
Use promo code "24074" to get your promotional price.

 Bekijk meerdere types van dit product via de

Produktcode. 10304720

  • 95.00 /

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers
This item is not returnable. View return policy

Beschrijving

Beschrijving

Lambda DNA digested with Mph1103I (NsiI), 0.7% agarose, 14 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'..A▵T G C A▵T...3'

3'..T▵A C G T▵A...5'

Conditions for 100% Activity:

  • 1X Buffer R: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Mph1103I is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: never overlaps —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —effect not determined

Compatible Ends:

  • Alw21I, PstI, SdaI, SduI
TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

10 U/μL
Mph1103I (NsiI)
Yes
No
Restriction Enzyme
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
10X Buffer R
37°C
1,000 units
Traditional Cloning
Product Suggestions

Product Suggestions

Video's
SDS
Documentatie

Documentatie

Certificates
Speciale aanbiedingen

Speciale aanbiedingen

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.