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Thermo Scientific™ GeneJET™ plantengenoom-DNA-zuiveringskit mini
Thermo Scientific GeneJET Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types.
Merk: Thermo Scientific™ K0792
791.51 EUR valid until 2025-08-24
Use promo code "24117" to get your promotional price.
This item is not returnable.
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Beschrijving
GeneJET Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types. It utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.
Thermo Scientific GeneJET Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types. It utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.
Highlights
• Fast—procedure completed in 30 min following cell lysis
• Convenient—protocol does not include clogging-prone lysate filtration step
• High yield—up to 32 μg of high quality DNA from 100 mg sample, including nuclear, mitochondrial, and chloroplast DNA
• Compatible—use with awide variety of samples (leaves, roots, sprouts and other parts of various plants)
The standard procedure takes less than 30 minutes following cell lysis and yields purified DNA greater than 30 kb in size. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and the age of the tissue sample. The typical yield from a standard tissue source (e. g. young wheat leaves) is 30 to 32 μg from 100 mg of tissue. Isolated DNA can be used directly in PCR, qPCR, southern blotting, and enzymatic reactions.
Applications
• Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
• PCR and qPCR
• FastDigest and conventional restriction digestion
• Southern blotting
Specificatie
| Spin Column | |
| Silica Spin Column | |
| Plants | |
| gDNA | |
| Not High-throughput Compatible (Manual) | |
| Room Temperature | |
| ≤32 μg per 100 mg tissue |
| • 6 x 1 mL RNase A; store at room temperature until opened, –20°C after opening • 120 mL Lysis Buffer A; room temperature • 15 mL Lysis Buffer B; room temperature • 40 mL Precipitation Solution; room temperature • 120 mL Plant gDNA Binding Solution; room temperature • 40 mL Wash Buffer I (concentrated); room temperature • 40 mL Wash Buffer II (concentrated); room temperature • 150 mL Elution Buffer; room temperature • 250 GeneJET Purification Columns with Collection Tubes; room temperature • 250 Collection Tubes; room temperature |
|
| Mini | |
| 200 μL | |
| PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization | |
| 250 Preps | |
| 30 min. (After sample homogenization) | |
| ≤100 mg of fresh/frozen ≤20 mg lyophilized |
Veiligheid en behandeling
Productidentificatie
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- GeneJET Plant Genomic DNA Purification Kit
Signaalwoord
- Waarschuwing
Gevarencategorie
- Acute toxiciteit Categorie 4
- Ernstig oogletsel/oogirritatie Categorie 2
- Huidcorrosie/-irritatie Indeling Categorie 2
Gevarenaanduiding
- H302-Schadelijk bij inslikken.
- H315-Veroorzaakt huidirritatie.
- H319-Veroorzaakt ernstige oogirritatie.
- H332-Schadelijk bij inademing.
Veiligheidsmaatregel
- P264-Na het werken met dit product grondig wassen.
- P270-Niet eten, drinken of roken tijdens het gebruik van dit product.
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
- P301+P310-NA INSLIKKEN: onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
- P301+P312-NA INSLIKKEN: bij onwel voelen een ANTIGIFCENTRUM / arts/ raadplegen.
- P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
- P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
- P310-Onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
- P330-De mond spoelen.
- P332+P313-Bij huidirritatie: een arts raadplegen.
- P337+P313-Bij aanhoudende oogirritatie: een arts raadplegen.
- P362+P364-Verontreinigde kleding uittrekken en wassen alvorens deze opnieuw te gebruiken.
- P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.
Aanvullende informatie
- MIXTURE LIST-Bevat: Sodium lauryl sulfate, Guanidine hydrochloride
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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