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Thermo Scientific™ DTT

Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.

Merk:  Thermo Scientific™ R0861

65.15 EUR valid until 2025-08-31
Use promo code "24157" to get your promotional price.



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Produktcode. 10699530

  • 70.50 / 5g

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Beschrijving

Beschrijving

DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro. DTT quantitatively reduces disulfide bonds and maintains monothiols in a reduced state .

At a final 0.1 M concentration, DTT is also widely used for disruption of protein disulfide bonds in SDS-polyacrylamide gel electrophoresis.

DTT quantitatively reduces disulfide bonds and maintains monothiols in a reduced state (1). At a final 0.1M concentration DTT is also widely used for disruption of protein disulfide bonds in SDS-polyacrylamide gel electrophoresis.
  • Free of endo-, exodeoxyribonucleases, ribonucleases and phosphatases

Recommended for:

Enzyme stabilization; Enzymatic reactions; Protein electrophoresis.

Note:

DTT is not stable in solution. Only freshly-made DTT solutions should be used. To prepare a 1M DTT solution, dissolve 1.55g of DTT powder in 10mL of deionized water (e.g., Water, nuclease-free).

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

0.1 M
DTT (Dithiothreitol)
5 g
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Veiligheid en behandeling

Veiligheid en behandeling

Productidentificatie
  • DTT

Signaalwoord
  • Waarschuwing

Gevarencategorie
  • Acute toxiciteit Categorie 4
  • Huidcorrosie/-irritatie Indeling Categorie 2
  • Ernstig oogletsel/oogirritatie Categorie 2

Gevarenaanduiding
  • H302-Schadelijk bij inslikken.
  • H315-Veroorzaakt huidirritatie.
  • H319-Veroorzaakt ernstige oogirritatie.

Veiligheidsmaatregel
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.
  • P264-Na het werken met dit product grondig wassen.
  • P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
  • P332+P313-Bij huidirritatie: een arts raadplegen.
  • P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
  • P337+P313-Bij aanhoudende oogirritatie: een arts raadplegen.
  • P301+P312-NA INSLIKKEN: bij onwel voelen een ANTIGIFCENTRUM / arts/ raadplegen.
  • P330-De mond spoelen.

Aanvullende informatie
  • MIXTURE LIST-Bevat: 2, 3-Butanediol, 1,4-dimercapto-, (R*,R*)-

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