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  7. 13385544

Gibco™ ExpiFectamine™ 293 Transfection Kit

Designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells.

Merk:  Gibco™ A14524

1252.10 EUR valid until 2025-12-31
Use promo code "24270" to get your promotional price.


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Produktcode. 13385544

  • 1346.00 / 1 liter

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Beschrijving

Beschrijving

The ExpiFectamine™ 293 Transfection Kit is a core component of the Expi293™ Expression System. It is designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells. The high-efficiency, cationic, lipid-based transfection reagent and transfection enhancers are designed to power the highest possible level of protein expression from Expi293F™ cells cultured in Expi293™ Expression Medium

  • Designed specifically for transfection of high density suspension cell culture, with matching transfection enhancers that boost transfection performance and protein expression
  • Achieves protein yields 2- to 10-fold higher than other transfection reagents used on high density 293 cell cultures
  • Employs the same transient expression protocols typically used in current low-density 293 suspension culture systems to easily switch from low density systems to the high yield, high density Expi293™ Expression System
  • Provides robust and reproducible transfection results, giving users greater confidence in their results
  • Scale transfections for culture volumes of less than 1 mL to greater than 10 liters, while maintaining equivalent volumetric protein yields

The ExpiFectamine™ 293 Transfection Kit is specifically designed to work with Expi293F™ cells in the Expi293™ Expression System. The transfection kit consists of the cationic lipid-based ExpiFectamine™ 293 Reagent and ExpiFectamine™ 293 Transfection Enhancers 1 and 2. The enhancers are added 18 to 22 hours post-transfection to boost transfection, cell viability, and protein expression. The protocol for use of ExpiFectamine™ 293 Transfection components is very similar to transfection protocols for other transient expression reagents, such as 293fectin™ and FreeStyle™ MAX.

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

Expi293F™
2.7 mL (3 x 0.9 mL) ExpiFectamine™ 293 Reagent
5 mL ExpiFectamine™ 293 Transfection Enhancer 1
50 mL ExpiFectamine™ 293 Transfection Enhancer 2

Sufficient for transfection of 1 L of culture

Store at 2°C to 8°C
Plasmid DNA
Transfection
Transfection Kit
For 1 L of culture
Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Established Cell Lines
Lipid-based Transfection
ExpiFectamine™
Cell Culture Grade
No
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Veiligheid en behandeling

Productidentificatie
  • ExpiFectamine 293 Transfection Kit

Signaalwoord
  • Waarschuwing

Gevarencategorie
  • Acute toxiciteit Categorie 4
  • Ernstig oogletsel/oogirritatie Categorie 2
  • Huidcorrosie/-irritatie Indeling Categorie 2
  • Dzia anie toksyczne na narzady docelowe Categorie 3

Gevarenaanduiding
  • H302-Schadelijk bij inslikken.
  • H312-Schadelijk bij contact met de huid.
  • H315-Veroorzaakt huidirritatie.
  • H319-Veroorzaakt ernstige oogirritatie.
  • H332-Schadelijk bij inademing.
  • H335-Kan irritatie van de luchtwegen veroorzaken.

Veiligheidsmaatregel
  • P261-Inademing van stof/rook/gas/nevel/damp/spuitnevel vermijden.
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.
  • P301+P312-NA INSLIKKEN: bij onwel voelen een ANTIGIFCENTRUM / arts/ raadplegen.
  • P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
  • P304+P340-NA INADEMING: de persoon in de frisse lucht brengen en ervoor zorgen dat deze gemakkelijk kan ademen.
  • P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.

Aanvullende informatie
  • MIXTURE LIST-Bevat: 3-Fluoro-4-nitrophenol

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For Research Use Only. Not for use in diagnostic procedures.