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Gibco™ Expi293™ Expression Medium
Expi293™ Expression Medium designed for growth, transfection of suspension-adapted human embryonic kidney (HEK) 293 cells.
Merk: Gibco™ A1435101
378.10 EUR valid until 2025-12-31
Use promo code "24331" to get your promotional price.
Waarschuwingen:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price
Beschrijving
Expi293™ Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression.
- Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
- Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine™ transfection reagent
- Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
- Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10 L in disposable bioreactors
- Does not contain phenol red
Expi293™ Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293™ Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.
Specificatie
| 293E (HEK), 293 (HEK), Expi293F™, FreeStyle™ 293-F Cells, 293F | |
| Animal Origin-free, Chemically-defined, Protein-free, Serum-free | |
| Liquid | |
| Serum-free | |
| High Glucose, GlutaMAX, Sodium Pyruvate, Sodium Bicarbonate, Dextrose, Pyridoxine HCl | |
| Expi293™ | |
| Room Temperature | |
| Bottle(s) | |
| Calcium, Magnesium, Sodium Phosphate | |
| Cell Culture Grade | |
| CO2 |
| Embryonic Kidney Cells | |
| 1 X | |
| Expression Medium | |
| Sterile | |
| No HEPES, No Phenol Red, No Hypoxanthine, No Thymidine, No Insulin, No Vitamin A, No 2-Mercaptoethanol | |
| 1 L | |
| < 1 EU/mL | |
| No antibiotics | |
| 275-295 mOsm/kg | |
| Store in the dark at +2°C to +8°C | |
| Suspension Cell Culture |
Veiligheid en behandeling
Productidentificatie
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- Expi293 Expression Medium
Signaalwoord
- Waarschuwing
Gevarencategorie
- Ernstig oogletsel/oogirritatie Categorie 2
- Huidcorrosie/-irritatie Indeling Categorie 2
- Dzia anie toksyczne na narzady docelowe Categorie 3
Gevarenaanduiding
- H315-Veroorzaakt huidirritatie.
- H319-Veroorzaakt ernstige oogirritatie.
- H335-Kan irritatie van de luchtwegen veroorzaken.
Veiligheidsmaatregel
- P261-Inademing van stof/rook/gas/nevel/damp/spuitnevel vermijden.
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
- P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
- P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
Aanvullende informatie
- MIXTURE LIST-Bevat: 4-Isopropoxybenzoicacid
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Product Title
For Research use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
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