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Thermo Scientific™ DyLight™ 488 Maleimide

Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of maleimide-activated DyLight Dyes.

Merk:  Thermo Scientific™ 46602


Produktcode. 10529103

  • 414.00 /

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Beschrijving

Beschrijving

Thermo Scientific™ Sulfhydryl-Reactive DyLight Dyes are maleimide-activated DyLight Fluors for fluorescent labeling of antibodies and other proteins to be used as probes for cellular imaging and other fluorescence detection methods.

The Thermo Scientific™ DyLight™ Dyes have absorption spectra ranging from 350 to 770nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor™ or Cy™ Dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight Reagents allows a high fluor-to-protein ratio without precipitation during conjugation.
The sulfhydryl-reactive dyes contain maleimide groups that react predominantly with free –SH groups at pH 6.5-7.5, forming a stable thioether bond. At pH 7, the maleimide group is ∼1000 times more reactive toward a free sulfhydryl than to an amine.1At pH values > 7.5, reactivity toward primary amines increases and hydrolysis of the maleimide group can occur.

Highlights:

  • Specific — maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
  • Efficient labeling methods — well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
  • Optimized antibody labeling procedure —complete protocol for IgG reduction and labeling and calculating the labeling efficiency

Applications:

  • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
  • Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies
TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

DyLight 488 Maleimide
Dye
Store desiccated at -20°C. Shipped at ambient temperature.
Maleimide
DyLight Fluors
518
1 mg
DMF (Dimethylformamide)
Thiol
DyLight™ 488
DyLight™
493
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Veiligheid en behandeling

Veiligheid en behandeling

Productidentificatie
  • DyLight 488 Maleimide,1 mg.

Signaalwoord
  • Gevaar

Gevarencategorie
  • Ernstig oogletsel/oogirritatie Categorie 1

Gevarenaanduiding
  • H318-Veroorzaakt ernstig oogletsel.

Veiligheidsmaatregel
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.
  • P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
  • P310-Onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.

Aanvullende informatie
  • MIXTURE LIST-Bevat: DyLight Reactive Dyes

SDS
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