missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Molecular Probes™ Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit
Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit
Merk: Molecular Probes™ C10632
494.46 EUR valid until 2025-06-30
Use promo code "24074" to get your promotional price.
Bekijk meerdere types van dit product via de
This item is not returnable.
View return policy
Beschrijving
The Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells compared to traditional BrdU methods. Newly synthesized DNA is analyzed using the 488 nm laser of the flow cytometer. The Click-iT™ Plus formulation is compatible with standard fluorophores, including R-PE and R-PE tandems, as well as fluorescent proteins.View selection guide for all Click-iT™ EdU and Click-iT™ Plus EdU assays for flow cytometry.
Multiplexable
The Click-iT™ Plus formulation provides increased multiplexibility compared to the original Click-iT™ EdU Flow Cytometry assays. Click-iT™ Plus EdU assays can be used in conjunction with R-PE and R-PE tandems, as well as fluorescent proteins such as GFP and mCherry, without loss of the accuracy or speed of the original Click-iT™ EdU assay.
An Advanced Method Giving You Results Superior to BrdU
The most accurate method of proliferation analysis is direct measurement of DNA synthesis. Originally, this was performed through incorporation of radioactive nucleosides. This method was replaced by antibody-based detection of the nucleoside analog bromodeoxyuridine (BrdU). The Click-iT™ Plus EdU Flow Cytometry assay is a novel alternative to the BrdU assay. EdU (5-ethynyl-2´-deoxyuridine) is a thymidine analog that is incorporated into DNA during active DNA synthesis. Detection is based on click chemistry, which is a copper-catalyzed covalent reaction between an azide and an alkyne. In this application, the alkyne is found in the ethynyl moiety of EdU, while the azide is coupled to the Alexa Fluor™ dye. Standard flow cytometry methods are used for determining the percentage of S-phase cells in the population.
Mild Conditions Allow Use with Cell Cycle Dyes and Antibodies
The small size of the dye azide allows for efficient detection of the incorporated EdU using mild conditions, while standard aldehyde-based fixation and detergent permeabilization are sufficient for the Click-iT™ Plus detection reagent to gain access to the DNA. This is in contrast to BrdU assays that require DNA denaturation (using HCl, heat, or digestion with DNase) to expose the BrdU so that it can be detected with an anti-BrdU antibody. Sample processing for the BrdU assay can result in signal alteration of the cell cycle distribution, as well as destruction of antigen recognition sites when using the HCl method. In contrast, the easy-to-use Click-iT™ Plus EdU assay is compatible with cell cycle dyes. The Click-iT™ Plus EdU assay can also be multiplexed with antibodies against surface and intracellular markers, as well as conjugates labeled with standard fluorophores including R-PE, R-PE tandems, and fluorescent proteins (GFP and mCherry).
Quick and Simple Protocol
The Click-iT™ Plus EdU protocol is based on the aldehyde fixation and detergent permeabilization steps for immunohistochemical antibody labeling. However, EdU is compatible with other fixation/permeabilization agents including saponin and methanol. In just five steps you’ll be ready to analyze your cell proliferation data:
1. Treat cells with EdU.
2. Fix and permeabilize cells.
3. Detect S-phase cells with Click-iT™ Plus detection cocktail for 30 min.
4. Wash once.
5. Analyze.
Results can be seen in as little as 60 minutes in some circumstances, but we recommend 90 minutes for all applications.
Specificatie
| Contains EdU (5-ethynyl-2' -deoxyuridine), Alexa Fluor™ 488 picolyl azide, anhydrous dimethylsulfoxide (DMSO), Click-iT™ fixative, Click-iT™ saponin-based permeabilization and wash buffer, Copper Protectant, and Click-iT™ EdU buffer additive. |
|
| Flow Cytometer | |
| Alexa Fluor™ 488 | |
| Tube(s) | |
| Click-iT | |
| Room Temperature |
| Fluorescence | |
| Flow Cytometry Assay Kit | |
| 488 | |
| Less hazardous | |
| 50 assays |
Veiligheid en behandeling
Productidentificatie
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- Click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit
Signaalwoord
- Gevaar
Gevarencategorie
- AQUATISCH GEVAAR OP LANGE TERMIJN Categorie 2 voor chronische toxiciteit
- Kankerverwekkendheid Categorie 1
- Ernstig oogletsel/oogirritatie Categorie 1
- Mutageniteit van geslachtscellen Categorie 1B
- Mutageniteit van geslachtscellen Categorie 2
- Giftigheid voor de voortplanting Categorie 2
- Sensibilisering van de huid Categorie 1
- Dzia anie toksyczne na narzady docelowe Categorie 2
Gevarenaanduiding
- H317-Kan een allergische huidreactie veroorzaken.
- H318-Veroorzaakt ernstig oogletsel.
- H340-Kan genetische schade veroorzaken.
- H341-Verdacht van het veroorzaken van genetische schade.
- H350-Kan kanker veroorzaken.
- H361-Kan mogelijks de vruchtbaarheid of het ongeboren kind schaden.
- H371-Kan schade aan organen.
- H412-Schadelijk voor in het water levende organismen, met langdurige gevolgen.
Veiligheidsmaatregel
- P201-Alvorens te gebruiken de speciale aanwijzingen raadplegen.
- P202-Pas gebruiken nadat u alle veiligheidsvoorschriften gelezen en begrepen heeft.
- P261-Inademing van stof/rook/gas/nevel/damp/spuitnevel vermijden.
- P264-Na het werken met dit product grondig wassen.
- P270-Niet eten, drinken of roken tijdens het gebruik van dit product.
- P273-Voorkom lozing in het milieu.
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
- P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
- P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
- P308+P313-NA (mogelijke) blootstelling: een arts raadplegen.
- P310-Onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
- P332+P313-Bij huidirritatie: een arts raadplegen.
- P391-Gelekte/gemorste stof opruimen.
- P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.
Aanvullende informatie
- MIXTURE LIST-Bevat: 5-ethynyl-2idine, Paraformaldehyde, Sulfuric acid,copper(2+) salt
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction