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Molecular Probes™ Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit
Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit
Merk: Molecular Probes™ C10424
506.52 EUR valid until 2025-06-30
Use promo code "24074" to get your promotional price.
This item is not returnable.
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Beschrijving
The Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. Newly synthesized DNA is analyzed using the 633/5 nm laser of the flow cytometer.View selection guide for all Click-iT™ EdU and Click-iT™ Plus EdU assays for flow cytometry.
An Advanced Method Giving You Results Superior to BrdU
The most accurate method of proliferation analysis is direct measurement of DNA synthesis. Originally, this was performed through incorporation of radioactive nucleosides, i.e., 3H-thymidine. This method was replaced by antibody-based detection of the nucleoside analog bromodeoxyuridine (BrdU). The Click-iT™ EdU Flow Cytometry Assay Kits are novel alternatives to the BrdU assay. EdU (5-ethynyl-2´-deoxyuridine) is a thymidine analog which is incorporated into DNA during active DNA synthesis. Detection is based on click chemistry: a copper catalyzed covalent reaction between an azide and an alkyne. In this application, the alkyne is found in the ethynyl moiety of EdU, while the azide is coupled to Alexa Fluor ™ 488, Alexa Fluor™ 647, or Pacific BlueTM dyes. Standard flow cytometry methods are used for determining the percentage of S-phase cells in the population (Fig 1).
Mild Conditions Allow Use with Cell Cycle Dyes and Antibodies
The advantages of Click-iT™ EdU labeling are readily evident while performing the assay (Fig 2). The small size of the dye azide allows for efficient detection of the incorporated EdU using mild conditions, while standard aldehyde-based fixation and detergent permeabilization are sufficient for the Click-iT™ detection reagent to gain access to the DNA. This is in contrast to BrdU assays that require DNA denaturation (using HCl, heat, or digestion with DNase) to expose the BrdU so that it may be detected with an anti-BrdU antibody. Sample processing for the BrdU assay can result in signal alteration of the cell cycle distribution as well as destruction of antigen recognition sites when using the HCl method. In contrast, the easy-to-use EdU cell proliferation kit is compatible with cell cycle dyes. This EdU assay kit can also be multiplexed with antibodies against surface and intracellular markers.
Quick and Simple Protocol
The Click-iT™ EdU protocol is based on the aldehyde fixation and detergent permeabilization steps for immunohistochemical antibody labeling, but EdU is compatible with other
fixation/permeabilization agents including saponin and methanol. In just five steps you’ll be ready to analyze your cell proliferation data:
Get Accurate Results Economically
By increasing the number of assays per kit, the Click-IT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit is less expensive than the traditional BrdU assays making them ideal for large experiments.
For research use only. Not intended for any human or animal therapeutic of diagnostic use.
Specificatie
| Contains EdU (5-ethynyl-2' -deoxyuridine), Alexa Fluor™ 647 azide, anhydrous dimethylsulfoxide (DMSO), Click-iT™ fixative, Click-iT™ saponin-based permeabilization and wash buffer, copper (II) sulfate,, and Click-iT™ EdU buffer additive. |
|
| Flow Cytometer | |
| Alexa Fluor™ 647 | |
| Tube(s) | |
| Click-iT | |
| Room Temperature |
| Fluorescence | |
| Flow Cytometry Assay Kit | |
| 650/670 | |
| Less hazardous | |
| 50 assays |
Veiligheid en behandeling
Productidentificatie
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- 647 Flow Cytometry Assay Kit
Signaalwoord
- Gevaar
Gevarencategorie
- AQUATISCH GEVAAR OP LANGE TERMIJN Categorie 3 voor chronische toxiciteit
- Kankerverwekkendheid Categorie 1
- Ernstig oogletsel/oogirritatie Categorie 1
- Mutageniteit van geslachtscellen Categorie 2
- Sensibilisering van de huid Categorie 1
- Dzia anie toksyczne na narzady docelowe Categorie 2
Gevarenaanduiding
- H317-Kan een allergische huidreactie veroorzaken.
- H318-Veroorzaakt ernstig oogletsel.
- H341-Verdacht van het veroorzaken van genetische schade.
- H350-Kan kanker veroorzaken.
- H371-Kan schade aan organen.
- H412-Schadelijk voor in het water levende organismen, met langdurige gevolgen.
Veiligheidsmaatregel
- P201-Alvorens te gebruiken de speciale aanwijzingen raadplegen.
- P261-Inademing van stof/rook/gas/nevel/damp/spuitnevel vermijden.
- P264-Na het werken met dit product grondig wassen.
- P270-Niet eten, drinken of roken tijdens het gebruik van dit product.
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
- P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
- P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
- P308+P313-NA (mogelijke) blootstelling: een arts raadplegen.
- P310-Onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
- P332+P313-Bij huidirritatie: een arts raadplegen.
- P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.
Aanvullende informatie
- MIXTURE LIST-Bevat: Paraformaldehyde
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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