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Invitrogen™ MEGAscript™ T7 Transcription Kit

Ultra-high yield technology for in vitro transcription

Merk:  Invitrogen™ AM1333

372.65 EUR valid until 2025-12-31
Use promo code "25339" to get your promotional price.


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Inclusief: T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II, Nuclease-free Water
This item is not returnable. View return policy

Beschrijving

Beschrijving

The high yields are achieved by modifying typical transcription reaction conditions so that very high nucleotide concentrations can be effectively used. The T7 Enzyme Mix and the 10X Reaction Buffer are specifically calibrated for each lot and RNA polymerase.

Using the MEGAscript T7 Kit:
The MEGAscript T7 Kit contains in vitro transcription reaction components and a control template. The kit will yield a total of 3 to 5mg of RNA (approximately 100μg of RNA or more per reaction) from the control template supplied with the kit. This corresponds to 400 to 650 moles of RNA for each mole of template. Smaller templates typically yield a lower mass and a higher molar yield of product. Novel transcription reaction conditions and a patented, high-yield technology allow the synthesis of 10 to 50 times the amount of RNA produced by conventional transcription reactions.

Applications:The MEGAscript T7 Kit is intended for the synthesis of large amounts of unlabeled or low specific activity RNA for a variety of uses, including in vitro translation, antisense/microinjection studies, and isolation of RNA binding proteins. In large-scale transcription reactions, the concentration of all four nucleotides is high, well above the Km for the enzyme. The MEGAscript T7 Kit typically yields over 10 times more RNA than conventional in vitro transcription reactions. The kit is not recommended for synthesis of high specific activity probes.

Accessory products:
MessageAmp™ aRNA amplification kits are powered by MEGAscript technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, use MEGAclear™ Kit (Mfr. No. AM1908) and the MEGAclear-96 Kit (Mfr.. No. AM1909).

RNA Yield:
100 μg

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Kit
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Ambion™, MEGAscript™
25 Reactions
Dry Ice
PCR Products, Linearized DNA plasmid
Synthetic mRNA, mRNA
mRNA, Synthetic mRNA
25 Reactions
T7 In Vitro Transcription kit
T7
100 μg
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Veiligheid en behandeling

Veiligheid en behandeling

Productidentificatie
  • MEGASCRIPT T7 KIT 25 RXNS EACH

Signaalwoord
  • Gevaar

Gevarencategorie
  • Acute toxiciteit Categorie 4
  • Kankerverwekkendheid Categorie 2
  • Ernstig oogletsel/oogirritatie Categorie 2
  • Giftigheid voor de voortplanting BCategorie 1A
  • Huidcorrosie/-irritatie Indeling Categorie 2
  • Dzia anie toksyczne na narzady docelowe Categorie 2

Gevarenaanduiding
  • H302-Schadelijk bij inslikken.
  • H315-Veroorzaakt huidirritatie.
  • H319-Veroorzaakt ernstige oogirritatie.
  • H351-Verdacht van het veroorzaken van kanker.
  • H360-Kan de vruchtbaarheid of het ongeboren kind schaden.
  • H373-Kan schade aan organen.

Veiligheidsmaatregel
  • P201-Alvorens te gebruiken de speciale aanwijzingen raadplegen.
  • P202-Pas gebruiken nadat u alle veiligheidsvoorschriften gelezen en begrepen heeft.
  • P260-Stof/rook/gas/nevel/damp/spuitnevel niet inademen.
  • P264-Na het werken met dit product grondig wassen.
  • P270-Niet eten, drinken of roken tijdens het gebruik van dit product.
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.
  • P301+P310-NA INSLIKKEN: onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
  • P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
  • P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
  • P308+P313-NA (mogelijke) blootstelling: een arts raadplegen.
  • P314-Bij onwel voelen een arts raadplegen.
  • P332+P313-Bij huidirritatie: een arts raadplegen.
  • P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.

Aanvullende informatie
  • MIXTURE LIST-Bevat: formamide, lithium chloride

SDS
Documentatie

Documentatie

Certificates
Speciale aanbiedingen

Speciale aanbiedingen

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For Research Use Only. Not for use in diagnostic procedures.