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Invitrogen™ Linear Acrylamide (5 mg/ml) (1 ml Tube)
Invitrogen Linear Acrylamide (5 mg/mL) is for use as a nucleic acid coprecipitant and is ideal for PCR and RT-PCR reactions since small amounts of contaminating nucleic acids present in other carriers could be amplified.
Merk: Invitrogen™ AM9520
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Beschrijving
Invitrogen Linear Acrylamide (5 mg/mL) is for use as a nucleic acid coprecipitant and is ideal for PCR and RT-PCR reactions since small amounts of contaminating nucleic acids present in other carriers could be amplified.
- Ideal for RT-PCR
- Increases pellet mass
- Quantitative recovery of low concentrations (ng/mL) of nucleic acid
- Prevents pellet loss in nuclease protection assays
What is a Coprecipitant?
Co-precipitants are inert substances used to aid recovery of nucleic acids during alcohol precipitations. While they can be used for precipitating large amounts of nucleic acids, they are essential for quantitative recovery of small amounts of nucleic acids in dilute solutions. Often, the use of such molecules is desirable for no other reason but visualization of the pelleted precipitate after centrifugation. Linear acrylamide offers advantages over other coprecipitants in that it is chemically synthesized and not treated with reagents (e.g., proteases) derived from biological sources. Therefore, it is not contaminated with biological material making it ideal for use upstream of RT-PCR. It has been shown to precipitate pictogram amounts of DNA fragments larger than 20 base pairs while failing to precipitate shorter fragments and free nucleotides. Linear acrylamide is useful for separating reaction products from unincorporated nucleotides and from most oligonucleotide primers. It should be used at a final working concentration of 10 to 20 μg/mL. Linear acrylamide will not interfere with A260/280 readings. Supplied in 5 x 1 mL tubes.
Informatie over bestelling
Shipping Condition: Dry Ice
Specificatie
| Acrylamide | |
| 5 mg/mL | |
| Ambion™ | |
| 5 x 1mL tubes | |
| Liquid |
| Store at –20°C. | |
| RNA Extraction | |
| Molecular Biology Grade | |
| Dry Ice |
Veiligheid en behandeling
Productidentificatie
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- Linear Acrylamide
Signaalwoord
- Gevaar
Gevarencategorie
- Kankerverwekkendheid Categorie 1B
- Mutageniteit van geslachtscellen Categorie 1B
- Giftigheid voor de voortplanting Categorie 2
- Sensibilisering van de huid Categorie 1
Gevarenaanduiding
- H317-Kan een allergische huidreactie veroorzaken.
- H340-Kan genetische schade veroorzaken.
- H350-Kan kanker veroorzaken.
- H361f-Wordt ervan verdacht de vruchtbaarheid te schaden.
Veiligheidsmaatregel
- P201-Alvorens te gebruiken de speciale aanwijzingen raadplegen.
- P202-Pas gebruiken nadat u alle veiligheidsvoorschriften gelezen en begrepen heeft.
- P261-Inademing van stof/rook/gas/nevel/damp/spuitnevel vermijden.
- P272-Verontreinigde werkkleding mag de werkruimte niet verlaten.
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
- P302+P352-BIJ CONTACT MET DE HUID: met veel water/ wassen.
- P308+P313-NA (mogelijke) blootstelling: een arts raadplegen.
- P333+P313-Bij huidirritatie of uitslag: een arts raadplegen.
- P362+P364-Verontreinigde kleding uittrekken en wassen alvorens deze opnieuw te gebruiken.
- P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.
Aanvullende informatie
- MIXTURE LIST-Bevat: Acrylamide
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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