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Thermo Scientific™ RevertAid™ First Strand cDNA-syntheseset

The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates.

Merk:  Thermo Scientific™ K1691

856.65 EUR valid until 2025-12-31
Use promo code "25339" to get your promotional price.

Produktcode. 15255146

  • 986.00 /

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Beschrijving

Beschrijving

The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. The enzyme maintains activity at 42–50°C and is suitable for synthesis of cDNA up to 13 kb. The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55°C.

First strand cDNA synthesized with this system can be directly used as a template in PCR or real-time PCR. It is also ideal as a first step for second strand cDNA synthesis or linear RNA amplification. Radioactively and non-radioactively labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays.

Features of the RevertAid RT Kit include:
• Full-length first strand cDNA up to 13 kb
• Optimum reaction temperature 42°C
• Complete kit includes all the components for RT reactions

Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

Informatie over bestelling

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

• RevertAid Reverse Transcriptase
• Ribolock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Random Hexamer Primer
• Nuclease-free water

Store at –20°C.
60 min.
42°C
RevertAid
Dry Ice
First-Strand cDNA
Separate components
Up to 13 kb
Kit
Reverse Transcription
500 reactions
Yes
Real Time PCR (qPCR)
500 Reactions
Reverse Transcription
RNA
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For Research Use Only. Not for use in diagnostic procedures.