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  7. Thermo Scientific™ Phire™ Tissue Direct PCR Master Mix
  8. 15264756

Thermo Scientific™ Phire™ Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Merk:  Thermo Scientific™ F170S


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Produktcode. 15264756

  • 211.00 / 250 reacties

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Beschrijving

Beschrijving

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, humans, fish, birds, and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue–derived inhibitors such as collagen, melanin and eumelanin (hair, skin), and myoglobin (muscle). The kit also includes DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.

Features

  • Direct PCR reduces time-consuming and expensive DNA purification steps
  • Available Dilution & Storage protocol allows multiple PCR reactions from a single sample
  • Specially engineered Phire DNA polymerase for extremely short PCR protocol times
  • Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing

Notes

  • The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

• 2X Phire Tissue Direct PCR Master Mix, 2 x 1.25 mL
• Dilution Buffer, 5 mL
• DNARelease Additive, 3 x 100 μL
• 25 μM Control Primer Mix, 40 μL
• Nuclease-free water, 2 x 1.25 mL
• 50 μg O'GeneRuler Express DNA Ladder

Store at -20°C.
Direct PCR
Fast
Direct PCR Master Mix
Wet or Dry Ice
2X
250 Reactions
SuperMix or Master Mix
DNA
Medium
Phire Hot Start II DNA Polymerase
Yes
250 reacties x 20 μL
Genotyping, Hot-start PCR
Built-In Hot Start
Blunt
Tissue
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Veiligheid en behandeling

Veiligheid en behandeling

Productidentificatie
  • Phire Tissue Direct PCR Master Mix/DNARelease Additive

Signaalwoord
  • Gevaar

Gevarencategorie
  • Sensibilisatie van de luchtwegen Categorie 1

Gevarenaanduiding
  • H334-Kan bij inademing allergie- of astmasymptomen of ademhalingsmoeilijkheden veroorzaken.

Veiligheidsmaatregel
  • P261-Inademing van stof/rook/gas/nevel/damp/spuitnevel vermijden.
  • P284-¢Bij ontoereikende ventilatie! adembescherming dragen.
  • P342+P311-Bij ademhalingssymptomen: Een ANTIGIFCENTRUM/arts/ raadplegen.
  • P304+P340-NA INADEMING: de persoon in de frisse lucht brengen en ervoor zorgen dat deze gemakkelijk kan ademen.
  • P501-Inhoud/verpakking afvoeren naar .

Aanvullende informatie
  • MIXTURE LIST-Bevat: Proteinase, tritirachium album serine

SDS
Documentatie

Documentatie

Certificates
Speciale aanbiedingen

Speciale aanbiedingen

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