Thermo Scientific™ Streptex™ Acid Extraction Kit

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Rapidly extract and identify streptococcal Lancefield group antigens from cultured organisms using Thermo Scientific™ Remel™ Streptex™ Acid Extraction Kit in conjunction with the Streptex™ Kit (ZL50/R30950501 and ZL61/R30164701). Lancefield showed that group-specific streptococcal antigens can be extracted in soluble form and identified by precipitation reactions with homologous antisera¹. The Streptex™ kit requires at least 10 minutes incubation at 37°C to extract the group antigen. Reagents supplied in this kit allow a more rapid extraction of group antigen.

Species of Streptococcus are classified based on their hemolytic properties (alpha and beta). Beta-hemolytic streptococci are further classified by Lancefield grouping. The majority of species of Streptococcus possess group-specific antigens which are usually carbohydrate structural components of the cell wall.

Group-specific antigens are extracted from streptococci by nitrous acid in a simple and short incubation step. The extract is then neutralised and antigens are detected and identified with suspensions of latex particles coated with group-specific antibodies.

• Ready-to-use: Reagent kit
• Rapid extraction: Within 1 minute.
• Ease of identification: Positive result is indicated by aggregation of latex particles.

The acid extraction kit provides reagents for the extraction of Lancefield group A, B, C, F and G at room temperature (18 – 30° C). Extraction is complete within 1 minute. Group D antigen cannot be extracted with these reagents.

The latex suspensions for groups A, B, C, D, F and G are supplied in the Streptex™ Kit (ZL50/R30950501 and ZL61/R30164701) or as individual suspensions.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.