Thermo Scientific™ Pierce™ zink-omkeerbare eiwitkleuringset

Beschrijving

Thermo Scientific™ Pierce Zinc Reversible Stain Kit provides rapid and highly sensitive detection of protein in polyacrylamide gels immediately after electrophoresis as well as erasability for subsequent transfer or band extraction.

The Pierce Zinc Reversible Stain Kit is an ideal option for in-gel protein detection preceding many downstream applications, because the zinc-stain is completely reversible. The white background is easily removed and the gel is restored to its original condition with the kit-supplied eraser solution or a Tris-glycine buffer. Afterwards, the proteins are free of any dyes or other contaminants and can be restained by another method, transferred to membrane for Western blotting or eluted from an excised gel piece.

Highlights:

• Sensitive – detect 0.25ng of protein per band in a mini gel

• Fast – the entire protocol is complete in 15 minutes

• Easy – ready-to-use stain and developer solutions are supplied, and no fixing or washing steps are required

• Gentle – no chemical modification of the proteins occurs; protein bands are visible because the background is stained, not the bands themselves

• Reversible – completely erase the stain from the gel to easily recover proteins or proceed with other methods

Includes:

Zinc stain, developer, and eraser solutions (500mL each)

Recommended for:

Staining preceding protein recovery for antibody generation or immunological detection; Staining before excising gel pieces for protein digestion and analysis by mass spectrometry; Staining before electrophoretic transfer to membrane for Western blotting; Quick purity checks before proceeding with purification or transfer for Western blotting