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Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
158.00 - 862.00
Specificatie
| Producttype | Substrate |
|---|---|
| Detectiemethode | Chemiluminescence, Fluorescence |
| Vorm | Liquid |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Product Line | Pierce™ |
| Produktcode | Merk | Aantal | Voldoende voor | Prijs | Hoeveelheid en beschikbaarheid | ||||
|---|---|---|---|---|---|---|---|---|---|
| Produktcode | Merk | Aantal | Voldoende voor | Prijs | Hoeveelheid en beschikbaarheid | ||||
|
11547910
|
Thermo Scientific™
32134 |
25 mL Kit | 250 cm2 of membrane |
158.00
25mL |
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|
11527271
|
Thermo Scientific™
32132X3 |
300 mL Kit | 3000 cm2 of membrane |
862.00
Pack of 3 |
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|
11517271
|
Thermo Scientific™
32132 |
100 mL Kit | 10 mini-gel blots, 1000 cm2 of membrane |
360.00
100mL |
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Beschrijving
Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.
Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. This product is sold as Pierce ECL 2 Substrate (Cat. #PI80196X3) through Fisher Scientific and other channels.
Features of ECL Plus Substrate:
- Easy to use—can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
- Higher sensitivity—detect targets down to the low-picogram level
- Longer signal duration—sustained light output for as long as 5 hours
- More imaging options—X-ray, CCD or laser-based imagers
- More affordable—high quality and performance at a lower price than other suppliers' similar substrates
Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.
Specificatie
| Substrate | |
| Liquid | |
| Pierce™ |
| Chemiluminescence, Fluorescence | |
| Nitrocellulose, PVDF | |
| Low picogram to mid-femtogram |
Veiligheid en behandeling
Productidentificatie
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- Pierce ECL Plus Western Blotting Substrate
Signaalwoord
- Gevaar
Gevarencategorie
- Kankerverwekkendheid Categorie 2
- Ernstig oogletsel/oogirritatie Categorie 2
- Ontvlambare vloeistof Categorie 2
- Dzia anie toksyczne na narzady docelowe Categorie 3
Gevarenaanduiding
- H225-Licht ontvlambare vloeistof en damp.
- H319-Veroorzaakt ernstige oogirritatie.
- H335-Kan irritatie van de luchtwegen veroorzaken.
- H351-Verdacht van het veroorzaken van kanker.
Veiligheidsmaatregel
- P201-Alvorens te gebruiken de speciale aanwijzingen raadplegen.
- P210-Verwijderd houden van warmte, hete oppervlakken, vonken, open vuur en andere ontstekingsbronnen. Niet roken.
- P241-Explosieveilige elektrische/ventilatie-/verlichtings-/ apparatuur gebruiken.
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
- P303+P361+P353-BIJ CONTACT MET DE HUID (of het haar): verontreinigde kleding onmiddellijk uittrekken. Huid met water afspoelen/afdouchen.
- P304+P340-NA INADEMING: de persoon in de frisse lucht brengen en ervoor zorgen dat deze gemakkelijk kan ademen.
- P403+P235-Op een goed geventileerde plaats bewaren. Koel bewaren.
- P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.
Aanvullende informatie
- MIXTURE LIST-Bevat: Ethanol
For Research Use Only. Not for use in diagnostic procedures.
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