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Thermo Scientific™ Pierce™ DAB substraatset

Chromogenic horseradish peroxidase (HRP) substrate for Western blot and tissue staining methods.

Merk:  Thermo Scientific™ 34002

135.85 EUR valid until 2025-05-31
Use promo code "25977" to get your promotional price.



Produktcode. 10006913

  • 146.00 /

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Beschrijving

Beschrijving

Thermo Scientific™ Pierce DAB and the DAB Substrate Kit enable chromogenic detection of horseradish peroxidase (HRP) activity based the action of 3,3'-diaminobenzidine (DAB) in blotting and tissue staining methods.

DAB (3,3'-diaminobenzidine tetrahydrochloride; MW = 214.1) reacts with HRP in the presence of peroxide to yield an insoluble brown-colored product at locations where peroxidase-conjugated antibodies are bound to samples. The brown precipitate is insoluble in alcohol and other organic solvents, making it an excellent substrate for immunohistochemical staining that requires the use of traditional counterstains and mounting media. The Pierce DAB Substrate Kit includes a 10X solution of DAB and Stable Peroxide Substrate Buffer. Crystalline DAB (powder) is also available.

Highlights:

  • HRP substrate – for detection of horseradish peroxidase activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples

  • Chromogenic – no special equipment needed for visualization; the DAB reaction produces brown bands or spots at sites of reaction with HRP-conjugated antibodies or probes

  • Package options – choose powder for custom formulation with buffer and peroxide or a complete kit of preformulated solutions for immediate use

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

Pierce DAB-substraatkit
Colorimetric
Nitrocellulose, PVDF
275 mL
Horseradish Peroxidase
25 Uses at 10 mL Each
Substrate
Liquid
Pierce™
HRP
Horseradish Peroxidase
Sufficient For: 25 uses at 10 mL each
• DAB (10X), 25 mL
• Stable Peroxide Buffer, 250 mL

Store kit at 4°C.
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Veiligheid en behandeling

Veiligheid en behandeling

Productidentificatie
  • Pierce DAB Substrate Kit

Signaalwoord
  • Gevaar

Gevarencategorie
  • Acute toxiciteit Categorie 3
  • Ontvlambare vloeistof Categorie 2
  • Dzia anie toksyczne na narzady docelowe Categorie 1

Gevarenaanduiding
  • H225-Licht ontvlambare vloeistof en damp.
  • H301-Giftig bij inslikken.
  • H311-Giftig bij contact met de huid.
  • H331-Giftig bij inademing.
  • H370-Veroorzaakt schade aan organen.

Veiligheidsmaatregel
  • P210-Verwijderd houden van warmte, hete oppervlakken, vonken, open vuur en andere ontstekingsbronnen. Niet roken.
  • P241-Explosieveilige elektrische/ventilatie-/verlichtings-/ apparatuur gebruiken.
  • P260-Stof/rook/gas/nevel/damp/spuitnevel niet inademen.
  • P280-Beschermende handschoenen/beschermende kleding/oog­bescherming/gelaatsbescherming dragen.
  • P301+P310-NA INSLIKKEN: onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
  • P303+P361+P353-BIJ CONTACT MET DE HUID (of het haar): verontreinigde kleding onmiddellijk uittrekken. Huid met water afspoelen/afdouchen.
  • P304+P340-NA INADEMING: de persoon in de frisse lucht brengen en ervoor zorgen dat deze gemakkelijk kan ademen.
  • P353-Huid met water afspoelen/afdouchen.
  • P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.

Aanvullende informatie
  • MIXTURE LIST-Bevat: methanol

SDS
Documentatie

Documentatie

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For Research Use Only. Not for use in diagnostic procedures.