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Thermo Scientific™ Pierce™ CN/DAB substraatset
Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.
Merk: Thermo Scientific™ 34000
164.72 EUR valid until 2025-05-31
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This item is not returnable.
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Beschrijving
Thermo Scientific™ Pierce CN/DAB Substrate Kit includes substrate and peroxide solutions for combined chloronaphthol- and diaminobenzidine-based detection of horseradish peroxidase (HRP) activity in blotting and tissue staining methods.
This kit combines two popular chromogenic peroxidase substrate compounds (4-CN and DAB) in a single, stable, 10X stock solution. This CN/DAB solution produces an intense dark black precipitate at sites of bound HRP-conjugated antibodies on probed blots and tissue samples. The Pierce CN/DAB Substrate Kit can be used in Western immunoblotting techniques as well as in dot blotting assays. The kit includes the 10X solution of CN/DAB and accompanying 1X Stable Peroxide Substrate Buffer.Highlights:
HRP substrate – for detection of horseradish peroxidase activity on solid media via the combined action of CN (4-chloro-1-naphthol) and DAB (3,3'-diaminobenzidine tetrahydrochloride)
Chromogenic – no special equipment needed for visualization; yields intense black bands that are easy to photograph
Easy-to-use kit – substrate stock solution and stable peroxide substrate buffer mean that there is no dry powders to measure and dissolve
Stable – store refrigerated at 4°C for at least one year
Includes:
10X solution of CN/DAB and the Pierce Stable Peroxide Substrate Buffer
Specificatie
| Substrate | |
| Liquid | |
| 275 mL | |
| Horseradish Peroxidase | |
| 25 Uses at 10 mL Each |
| Colorimetric | |
| Pierce™ | |
| HRP | |
| Horseradish Peroxidase | |
| Sufficient For: 25 uses at 10 mL each • CN/DAB (10X), 25 mL • Stable Peroxide Buffer, 250 mL Store at 4°C. |
Veiligheid en behandeling
Productidentificatie
Signaalwoord
Gevarencategorie
Gevarenaanduiding
Veiligheidsmaatregel
Aanvullende informatie
- CN/DAB Substrate Kit
Signaalwoord
- Gevaar
Gevarencategorie
- Acute toxiciteit Categorie 3
- Ontvlambare vloeistof Categorie 2
- Dzia anie toksyczne na narzady docelowe Categorie 1
Gevarenaanduiding
- H225-Licht ontvlambare vloeistof en damp.
- H301-Giftig bij inslikken.
- H311-Giftig bij contact met de huid.
- H331-Giftig bij inademing.
- H370-Veroorzaakt schade aan organen.
Veiligheidsmaatregel
- P210-Verwijderd houden van warmte, hete oppervlakken, vonken, open vuur en andere ontstekingsbronnen. Niet roken.
- P241-Explosieveilige elektrische/ventilatie-/verlichtings-/ apparatuur gebruiken.
- P260-Stof/rook/gas/nevel/damp/spuitnevel niet inademen.
- P280-Beschermende handschoenen/beschermende kleding/oogbescherming/gelaatsbescherming dragen.
- P301+P310-NA INSLIKKEN: onmiddellijk een ANTIGIFCENTRUM/arts/ raadplegen.
- P304+P340-NA INADEMING: de persoon in de frisse lucht brengen en ervoor zorgen dat deze gemakkelijk kan ademen.
- P305+P351+P338-BIJ CONTACT MET DE OGEN: voorzichtig afspoelen met water gedurende een aantal minuten; contactlenzen verwijderen, indien mogelijk; blijven spoelen.
- P405-Achter slot bewaren.
- P501b-Inhoud/verpakking afvoeren naar verwerken volgens de plaatselijke/regionale/nationale/internationale voorschriften.
Aanvullende informatie
- MIXTURE LIST-Bevat: methanol
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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