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OPTIZYME™ SphI (PaeI), Fisher BioReagents™

Merk:  Fisher BioReagents BP8029-1

Produktcode. 11964792

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Beschrijving

Beschrijving

5'...G C A T G^C...3'
3'...C^G T A C G...5'

Supplied with: 10X OPTIZYME Buffer 1
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 1: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 100%
  • Buffer 2: 50 - 100%
  • Buffer 3: 0 - 20%
  • Buffer 4: 50 - 100%
  • Buffer 5: 0 - 20%
Storage Buffer:
  • 10mM potassium-phosphate (pH 7.0 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.5mg/ml BSA and 50% (v/v) glycerol
Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with SphI.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of SphI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Compatible Ends: Hin1II, XceI
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: May overlap - no effect
    • 5'...m5C GCATGm5C G...3'
  • 3'... Gm5CGTAC Gm5C...5'
  • (No effect on cleavage)
  • EcoKI: Never overlaps - no effect
  • EcoBI: May overlap - blocked
    • 5'...TGm6A(N)5GCA TGCT...3'
  • 3'...AC T(N)5CGTm6ACGA...5'
  • (Cleavage is blocked)

TRUSTED_SUSTAINABILITY
Specificatie

Specificatie

10 U/μL
37°C
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with SphI
500 U
SphI
10X OPTIZYME™ Buffer 1
7.4
10mM Potassium Phosphate (pH 7 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton™ X-100, 0.5mg/mL BSA, 50% Glycerol
GCATG.C
Liquid
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  • OPTIZYME

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